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Control by phytochrome of the appearance of ribulose-1,5-bisphosphate carboxylase and the mRNA for its small subunit

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Abstract

We have measured levels of ribulose-1,5-bisphosphate carboxylase (RuBPCase) and levels of in-vitro-translatable mRNA for the small subunit (SSU) of RuBPCase up to 96 h after sowing in mustard (Sinapis alba L.) cotyledons, in order to investigate to what extent the rate of enzyme synthesis is related to the level of SSU-mRNA. Both enzyme and mRNA level are controlled strongly by phytochrome, but the rate of RuBPCase accumulation was found to be unrelated to the level of translatable SSU-mRNA. As an example, it was found that the amount of SSU-mRNA in far-red light (FR)-grown mustard seedlings doubles between 54 and 84 h after sowing while the rate of RuBPCase accumulation remains constant over this period. Since the holoenzyme shows zero turnover during this period it is concluded that the rate of enzyme synthesis remains constant although the level of SSU-mRNA increases strongly. Following an FR→dark transition, with different levels of physiologically active phytochrome (Pfr) established at the end of the light period, no correlation was found between the time course of mRNA levels in darkness and the rate of enzyme synthesis. Rather, the data indicate that there is at least one translational or post-translational regulatory step which is also phytochrome-dependent. It is concluded that coarse control of the appearance of translatable SSU-mRNA is essential for RuBPCase to appear at a high rate but that fine tuning by phytochrome of the actual appearance of RuBPCase is not transcriptional.

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Abbreviations

cD:

continuous darkness

cFR:

continuous far-red light

FR:

far-red light (3.5 W·m-2)

LHCP:

lightharvesting chlorophyll a/b-binding protein of photosystem II

LSU:

large subunit of RuBPCase

Pfr:

phytochrome (far-red absorbing)

Pr:

phytochrome (red absorbing)

R:

red light (6.8 W·m-2)

RG9-light:

long-wavelength far-red light obtained with RG9 glass filter

RuBPCase:

ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39)

SSU:

small subunit of RuBPCase

ϕλ:

\(\frac{{[Pfr]_\lambda }}{{[\Pr + Pfr]}}\)

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  1. Biologisches Institut II der Universität, Schänzlestrasse 1, D-7800, Freiburg i.Br., Federal Republic of Germany

    C. Schuster, R. Oelmüller & H. Mohr

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  1. C. Schuster
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  2. R. Oelmüller
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  3. H. Mohr
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Schuster, C., Oelmüller, R. & Mohr, H. Control by phytochrome of the appearance of ribulose-1,5-bisphosphate carboxylase and the mRNA for its small subunit. Planta 174, 426–432 (1988). https://doi.org/10.1007/BF00959531

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