Abstract
Protoplasts from suspension-culturedDaucus carota L. cells, when fixed and incubated with fluorescein conjugates of the fucosyl-specific lectinUlex europaeus agglutinin I, exhibited the following pattern of labeling: plasma membranes were not marked, but striking halos of fluorescence appeared around the periphery of all nucleic. Identical observations were made with protoplasts fromVicia faba L. leaves,Pisum sativum L. epicotyls,Zea mays L. roots andGlycine max L. cell suspensions, as well as with nucleic in cell-free preparations from the same sources. These results indicate that in a broad spectrum of angiosperm cells, fucose residues are associated with the nuclear envelope. The relationship of this finding in plant cells to recent discoveries regarding nuclear glycoconjugates in animal cells remains to be explored.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- Con A:
-
Concanavalin A
- PNA:
-
peanut agglutinin
- UEA I:
-
Ulex europaeus agglutinin I
References
Banks, M.S., Evans, P.K. (1976) A comparison of the isolation and culture of mesophyll protoplasts from severalNicotiana species and their hybrids. Plant Sci. Lett.7, 409–416
Chrispeels, M.J. (1984) Biosynthesis, processing and transport of storage proteins and lectins in cotyledons of developing legume seeds. Phil. Trans. R. Soc. London Ser. B304, 309–322
Datta, N., Chen, Y.-R., Roux, S.J. (1985) Phytochrome and calcium stimulation of protein phosphorylation in isolated pea nucleic. Biochem. Biophys. Res. Commun.128, 1403–1408
Davis, L.I., Blobel, G. (1986) Identification and characterization of a nuclear pore complex protein. Cell45, 699–709
Davis, L.I., Blobel, G. (1987) Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway. Proc. Natl. Acad. Sci. USA84, 7552–7556
Eriksson, T. (1965) Studies on the growth requirements and growth measurements of cell cultures ofHaplopappus gracilis. Physiol. Plant.18, 976–993
Frederick, S.E., Nies, B., Gruber, P.J. (1981) An ultrastructural search for lectin-binding sites on surfaces of spinach leaf organelles. Planta152, 145–152
Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res.50, 151–158
Gregoire, M., Hernandez-Verdun, D., Bouteille, M. (1984) Visualization of chromatin distribution in living PTO cells by Hoechst 33342 fluorescent staining. Exp. Cell Res.152, 38–46
Gronwald, J.W., Leonard, R.T. (1982) Isolation and transport properties of protoplasts from cortical cells of corn roots. Plant Physiol.70, 1391–1395
Gruber, P.J. (1987) Lectins. In: Handbook of plant cytochemistry, vol. II, pp. 45–63, Vaughn, K.C., ed. CRC Press, Boca Raton, Fla., USA
Gruber, P.J., Glimelius, K., Eriksson, T., Frederick, S.E. (1984) Interactions of galactose-binding lectins with plant protoplasts. Protoplasma121, 34–41
Gruber, P.J., Sweeney, K.A., Frederick, S.E. (1985) Lectin binding by the nuclear envelope inDaucus carota protoplasts. (Abstr.) Plant Physiol.77, Suppl., 73
Herman, E.M., Shannon, L.M., Chrispeels, M.J. (1986) The Golgi apparatus mediates the transport and post-translational modification of protein body proteins. In: Molecular biology of seed storage proteins and lectins, pp. 163–173, Shannon, L.M., Chrispeels, M.J., eds. American Society of Plant Physiologists, Rockville, Md., USA
Holt, G.D., Snow, C.M., Senior, A., Haltiwanger, R.S., Gerace, L., Hart, G.W. (1987) Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J. Cell Biol.104, 1157–1164
Hori, H., Elbein, A.D. (1983) Processing of N-linked oligosaccharides in soybean cultured cells. Arch. Biochem. Biophys.220, 415–425
Kan, F.W.K., Pinto da Silva, P. (1986) Preferential association of glycoproteins to the euchromatin regions of cross-fractured nuclei is revealed by fracture-label. J. Cell Biol.102, 576–586
Kornfeld, R., Kornfeld, S. (1985) Assembly of asparagine linked oligosaccharides. Annu. Rev. Biochem.54, 631–664
Saxena, P.K., Fowke, L.C., King, J. (1985) An efficient procedure for isolation of nuclei from plant protoplasts. Protoplasma128, 184–189
Snow, C.M., Senior, A., Gerace, L. (1987) Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J. Cell Biol.104, 1143–1156
Sturm, A., Johnson, K.D., Szumilo, T., Elbein, A.D., Chrispeels, M.J. (1987) Subcellular localization of glycosidases and glycosyltransferases involved in the processing of N-linked oligosaccharides. Plant Physiol.85, 741–745
Tallman, G., Reeck, G.R. (1980) Isolation of nucleic from plant protoplasts without the use of a detergent. Plant Sci. Lett.18, 271–275
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gruber, P.J., Sweeney, K.A. & Frederick, S.E. The detection of fucose residues in plant nuclear envelopes. Planta 174, 298–304 (1988). https://doi.org/10.1007/BF00959513
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00959513