Abstract
Methods are described for the isolation of functional protoplasts from secondary pulvinus tissue (flexor and extensor) and from leaf mesophyll tissue of primary leaves ofPhaseolus coccineus L. Integrity of the protoplasts was shown by vital staining and their ability to evolve oxygen in the light. Extensor-cell protoplasts increased their volume for up to 60% upon addition of 10 mM KCl. This K+-induced swelling was accompanied by increased rates of proton extrusion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase inBeta vulgaris. Plant Physiol.24, 1–15
Assmann, S.M., Simoncini, L., Schroeder, J.I. (1985) Blue light activates electrogenic ion pumping in guard cell protoplasts ofVicia faba. Nature318, 285–287
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem.72, 248–255
Brauner, M. (1933) Untersuchungen über die Lichtturgorreaktionen des Primärblattgelenkes vonPhaseolus multiflorus. Planta18, 288–337
Bünning, E. (1959) Tagesperiodische Bewegungen. In: Handbuch der Pflanzenphysiologie, vol. XVII/1: Physiologie der Bewegungen, pp. 579–656, Ruhland, W., ed. Springer, Berlin Göttingen Heidelberg
Fitzsimons, P.J., Weyers, J.D.B. (1983) Separation and purification of protoplast types fromCommelina communis L. J. Exp. Bot.34, 55–66
Freudling, C., Mayer, W.-E., Gradmann, D. (1980) Electrical membrane properties and circadian rhythm in extensor cells of the laminar pulvini ofPhaseolus coccineus L. Plant Physiol.65, 966–968
Gorton, H.L., Satter, R.L. (1984a) Extensor and flexor protoplasts fromSamanea pulvini. I. Isolation and initial characterization. Plant Physiol.76, 680–684
Gorton, H.L., Satter, R.L. (1984b) Extensor and flexor protoplasts fromSamanea pulvini. II. X-ray analysis of potassium, chlorine, sulfur, phosphorus and calcium. Plant Physiol.76, 685–690
Hampp, R., Goller, M., Füllgraf, H. (1984) Determination of compartmented metabolite pools by a combination of rapid fractionation of oat mesophyll protoplasts and enzymic cycling. Plant Physiol.75, 1017–1021
Hampp, R., Mehrle, W., Zimmermann, U. (1986) Assay of photosynthetic oxygen evolution from single protoplasts. Plant Physiol.81, 854–858
Hampp, R., Schnabl, H. (1984) Adenine and pyridine nucleotide status of isolatedVicia guard cell protoplasts during K+-induced swelling. Plant Cell Physiol.25, 1233–1239
Hampp, R., Ziegler, H. (1980) On the use ofAvena protoplasts to study chloroplast development. Planta147, 485–494
Hosokawa, Y., Kiyosawa, K. (1983) Diurnal K+ and anion transport inPhaseolus pulvinus. Plant Cell Physiol.24, 1065–1072
Iglesias, A., Satter, R.L. (1983) H+ fluxes in excisedSamanea motor tissue. II. Rhythmic properties. Plant Physiol.72, 570–572
Kottmeier, C., Schnabl, H. (1986) The Km-value of phosphoenolpyruvate carboxylase as an indicator of the swelling state of quard cell protoplasts. Plant Sci.43, 213–217
Mayer, W.-E. (1977) Kalium- und Chloridverteilung im Laminargelenk vonPhaseolus coccineus L. während der circadianen Blattbewegung im tagesperiodischen Licht-Dunkelwechsel. Z. Pflanzenphysiol.83, 127–135
Mayer, W.-E., Flach, D., Raju, M.V.S., Starrach, N., Wiech, E. (1985) Mechanics of circadian pulvini movements inPhaseolus coccineus L. Shape and arrangement of motor cells, micellation of motor cell walls, and bulk moduli of extensibility\((\bar \varepsilon _s )\). Planta163, 381–390
Mosebach, G. (1940) Untersuchungen über die tagesperiodische Bewegung der Blattgelenke vonPhaseolus. Jahrb. wiss. Bot.89, 20–88
Outlaw, Jr., W.H. (1983) Current concepts on the role of potassium in stomatal movements. Physiol. Plant.59, 302–311
Pfeffer, W. (1875) Die periodischen Bewegungen der Blattorgane. Verlag von Wilhelm Engelmann, Leipzig
Racusen, R.H., Satter, R.L. (1975) Rhythmic and phytochrome-regulated changes in transmembrane potential inSamanea pulvini. Nature255, 408–410
Raschke, K. (1979) Movements of stomata. In: Encyclopedia of plant physiology, N.S., vol. 7: Physiology of movements, pp. 383–441, Haupt, W., Feinleib, M.E., eds. Springer Verlag, Berlin Heidelberg New York
Raschke, K., Humble, G.D. (1973) No uptake of anions required by opening stomata ofVicia faba: guard cells release hydrogen ions. Planta115, 47–57
Ruge, W.A., Hampp, R. (1988) Leaf movements of nyctinastic plants-a review. Life Sci. Adv.6B, in press
Satter, R.L. (1979) Leaf movements and tendril curling. In: Encyclopedia of plant physiology N.S., vol. 7: Physiology of movements, pp. 442–484, Haupt, W., Feinleib, M.E., eds. Springer Verlag, Berlin Heidelberg New York
Satter, R.L., Galston, A.W. (1981) Mechanism of control of leaf movements. Annu. Rev. Plant Physiol.32, 83–110
Schnabl, H. (1980) Anion metabolism as correlated with the volume changes of guard cell protoplasts. Z. Naturforsch. Teil C,35, 621–626
Schnabl, H., Bornman, C.H., Ziegler, H. (1978) Studies on isolated starch containing (Vicia faba) and starch deficient (Allium cepa) guard cell protoplasts. Planta143, 33–39
Schnabl, H., Elbert, C., Krämer, G. (1982) The regulation of the starch-malate balances during volume changes of guard cell protoplasts. J. Exp. Bot.33, 996–1003
Schnabl, H., Kottmeier, C. (1984) Properties of phosphoenolpyruvate carboxylase in desalted extracts from isolated guard-cell protoplasts. Planta162, 220–225
Schroeder, J.I., Raschke, K. (1986) Voltage-dependent gating of potassium-selective channels in guard cell protoplasts ofVicia faba. (Abstr.) Plant Physiol80, Suppl. 64
Shimazaki, K., Gotow, K., Kodo, N. (1982) Photosynthetic properties of guard cell protoplasts fromVicia faba L. Plant Cell Physiol.23, 871–879
Shimazaki, K., Iino, M., Zeiger, E. (1986) Blue light-dependent proton extrusion by guard-cell protoplasts ofVicia faba. Nature319, 324–326
Sze, H. (1984) H+-translocating ATPases of the plasma membrane and tonoplast of plant cells. Physiol Plant.61, 683–691
Starrach, N., Flach, D., Mayer, W.-E. (1985) Activity of fixed negative charges of isolated extensor cell walls of the laminar pulvinus of primary leaves ofPhaseolus. J. Plant Physiol.120, 441–455
Vienken, J., Zimmermann, U., Ganser, R., Hampp, R. (1983) Vesicle formation during electro-fusion of mesophyll protoplasts ofKalanchoe daigremontiana. Planta157, 331–335
Woolhouse, H.W. (1984) The biochemistry and regulation of senescence in chloroplasts. Can. J. Bot.62, 2934–2942
Zeiger, E. (1983) The biology of stomatal guard cells. Annu. Rev. Plant Physiol.34, 441–475
Zeiger, E., Hepler, P.K. (1976) Production of guard cell proto-plasts from onion and tobacco. Plant Physiol.58, 492–498
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Erath, F., Ruge, W.A., Mayer, W.E. et al. Isolation of functional extensor and flexor protoplasts fromPhaseolus coccineus L. pulvini: potassium induced swelling. Planta 173, 447–452 (1988). https://doi.org/10.1007/BF00958956
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00958956