Abstract
A recombinant strain (D41) between phage T2 and T4 was isolated which possessed the T2 region of the genome between genes 32 and 39 and both the T4 genesαgt + andβgt + for glucosyltransferase. D41 was crossed with T4amber mutants in the genes for early functions and in some genes for late funcitions. The progeny of the crosses was examined for the frequency of theam + markers from D41. Genes 32, 60 and 39 in the T2 region of the recombinant strain were as sensitive to exclusion as those in standard-type T2. The T4 glucosylation of the DNA of these T2 genes did not protect them against partial exclusion by T4. However, genes in the region from gene 56 to 55 in the recombinant were resistent to exclusion. In standard-type T2 this region of the genome is sensitive to partial exclusion by T4. There are at least four exclusion sensitive sites in T2: one near gene 32, one near gene 60, one linked to gene 56 and one between genes 42 and 55.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams M. H. (1959).Bacteriophages. Interscience Publ. Inc. N.Y.
Bertani L. E. (1957). The effect of inhibition of protein synthesis on the establishment of lysogeny.Virology 4: 53–71.
Edgar R. S. & W. B. Wood (1966). Morphogenesis of bacteriophage T4 in extracts of mutant-infected cells.Proc. natl. Acad. Sci. 55: 498–505.
Fielding P. E. & M. R. Lunt (1970). The relation between breakdown of superinfecting virus deoxyribonucleic acid and temporal exclusion induced by T4 and T5 bacteriophages.J. gen. Virol. 6: 333–342.
Georcopoulos C. P. (1968). Location of glucosyl transferase genes on the genetic map of phage T4.Virology 34: 364–366.
De Groot B. (1966). Partial exclusion of bacteriophage T2 by T4: An early function of the second linkage group.Genetica 37: 37–51.
De Groot B. (1967a). The bar-properties, in particular glucosylation of deoxyribonucleic acid, in crosses of bacteriophages T2 and T4.Genet. Res. 9: 149–158.
De Groot B. (1967b). Polarized exclusion of bacteriophage T2 in crosses with T4.Genet. Res. 10: 13–20.
Hattman S. & T. Fukasawa (1963). Host-induced modification of T-even phages due to defective glucosylation of their DNA.Proc. Natl. Acad. Sci. 50: 297–300.
Hershey A. D. & R. Rotmann (1948). Linkage among genes controlling inhibition of lysis in a bacterial virus.Proc. Natl. Acad. Sci. 34: 89–96.
Kornberg S. R., S. B. Zimmerman & A. Kornberg (1961). Glucosylation of deoxyibonucleic acid by enzymes from bacteriophage-infectedEscherichia coli.J. Biol. Chem. 236: 1487–1493.
Masamune Y. (1968). The effect of ultraviolet light on the intracellular stability of the DNA of bacteriophage T2 and T4.Molec. gen. Genet. 101: 99–111.
Pees E. (1970). Bacteriophage T4 mutants unable to exclude gene 56 of T2 from the progeny of crosses.Mutation Research 9: 345–348.
Shedlovsky A. & S. Brenner (1963). A chemical basis for the host-induced modification of T-even bacteriophages.Proc. Natl. Acad. Sci. 50: 300–305.
Streisinger G. (1956). The genetic control of host range and serological specificity in bacteriophages T2 and T4.Virology 2: 377–387.
Streisinger G., F. Mukai & S. W. Kim (1959). The role of DNA glucose in exclusion of T2 by\(\overline {T2} \).Carnegie Institution of Washington Year Book 58: 432–433.
Streisinger G & J. Weigle (1956). Properties of bacteriophages T2 and T4 with unusual inheritance.Proc. Natl. Acad. Sci. 42: 504–506.
Wood W. B. (1966). Host specificity of DNA produced byEscherichia coli: Bacterial mutations affecting the restriction and modification of DNA.J. Mol. Biol. 16: 118–133.
Author information
Authors and Affiliations
Additional information
This investigation was carried out partially within the frame of the Association between Euratom and the University of Leiden, contract nr. 052-64-1-BIAN.
Rights and permissions
About this article
Cite this article
Pees, E., de Groot, B. Partial exclusion of genes of bacteriophage T2 with T4-glucosylated DNA in crosses with bacteriophage T4. Genetica 41, 541–550 (1970). https://doi.org/10.1007/BF00958933
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00958933