Summary
The presence of alkaline phosphatase was investigated in slide preparations and smears of lymph nodes with the azo dye method.
In the case of normal or hyperplastic lymph nodes, capillaries and arterioles are regularly and most strongly positive; the phosphatase activity in the endothelium of the venules, the larger blood vessels and the lymph vessels is irregular. One occasionally finds a few positive fibroblasts in the capsule and trabeculae. In addition, the pulpa sometimes shows a reaction in the region of the sinus, especially of the marginal sinus, and in the region of the germinal centers which appears to be associated with fibers. The activity in the region of the germinal centers is most fully developed with severe follicular lymphatic hyperplasia. A considerable degree of alkaline phosphatase activity can be noted at the edge of tubercles and tubercular caseous foci and in the scarified areas of the lympho-granulomatosis.
In lymph node smears, elements interpreted as large reticulum cells were found to be about 16% phosphatase active; in part these cells are for certain vascular endothelium and fibroblasts.
In the smears, we also found small lymphoid reticulum cells which gave a clear cut phosphatase reaction. No alkaline phosphatase activity could be demonstrated in the tissue mast cells, at least, that is, in their granula.
Phosphatase activity could be clearly demonstrated in slide preparations as in smears in a part of the cells from 2 lymphosarcomas.
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Lennert, K., Löffler, H. & Leder, L.D. Fermenthistochemische Untersuchungen des Lymphknotens. Virchows Arch. path Anat. 334, 399–418 (1961). https://doi.org/10.1007/BF00956999
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DOI: https://doi.org/10.1007/BF00956999