Optimization of the chromatographic process in liquid chromatography

Conclusions

• 1. Optimization of the Chromatographic process was examined in liquid chromatography with respect to the choice of the Chromatographic mode determined by mutually coordinated optimum values of the pressure differential in the Chromatographie system, the column length, and the sorbent grain diameter (mobile phase channels in open capillary columns and porous films).

• 2. The necessity of studying optimization with respect to the rate, efficiency, and sensitivity of the chromatographic analysis was demonstrated.

• 3. The necessity of characterizing each optimum chromatographic mode by the values of two fundamental optimization parameters was substantiated: the dimensionless elution rate (Ν) and its product by the dimensionless height equivalent to a theoretical plate (h): k=hΝ.

• 4. An optimization nomogram was plotted which makes it easy to find the corresponding values of the sorbent grain diameter d (capillaries), column length L, pressure differential in the column AP, time of analysis t, and efficiency of the analysis N; it also permits obtaining coordinated values of d, L, δP, t, and N for any other system if the optimum values of the fundamental optimization parameters Ν and k are determined for the system.