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Journal of Biological Physics

, Volume 22, Issue 2, pp 113–124 | Cite as

Two stable electron-conformational states of photoactivated reaction center and their observation in primary donor recovery kinetics

  • Alexander O. Goushcha
  • Natalja M. Berezetska
  • Marina T. Kapoustina
  • Valery N. Kharkyanen
Article

Abstract

We investigated the peculiarity of primary donor recovery kinetics in the reaction centers from the purple bacteriaRb. Sphaeroides at low levels of their cw photoactivation. A pronounced biphasity of the relaxation kinetics was found for the total light activating intensity <5×1012 quanta·cm-2·s-1. The effect was attributed to strong dependence of an electron transfer rate constant for the reactionP+Q infA pup- QBP+QAQ infB sup- upon the RC conformational state controlled by the light. We showed the existence of two different electron-conformational states for the photoexcited RC. The first reveals itself at low intensity of cw photoactivation while the second becomes actual under the intensity >5×1012 quanta · cm-2 · s-1.

Key words

Electron-conformational interaction Reaction center Rb. Sphaeroides 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Alexander O. Goushcha
    • 1
  • Natalja M. Berezetska
    • 1
  • Marina T. Kapoustina
    • 1
  • Valery N. Kharkyanen
    • 1
  1. 1.Division of Physics of Biological SystemsIPh. Nat. Acad. Sci. UkraineKyivUkraine

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