Advertisement

Histochemistry

, 83:245 | Cite as

Cytochemical localisation of acid phosphatase in the phagocytising conjunctival epithelium of the guinea pig

  • S. Latkovic
Article

Summary

The ultrastructural localisation of acid phosphalase activity was investigated on the guinea pig conjunctival epithelium incubated in vivo with a suspension of latex spheres. Deposits of acid phosphatase reaction product were concentrated on the elements of GERL, the phagocytic vacuoles, and the cell membrane. Acid phosphatase activity in GERL was intense in basal and suprabasal cells and decreased towards the superficial cells. Phagosomes containing latex spheres and reaction product of acid phosphatase were observed mainly in the centrospheral region of the superficial and intermediate epithelial cells. Acid phosphatase activity in phagocytising cells was not increased as compared to that in non-phagocytising cells. The observations indicate that existing acid phosphatase in unstimulated conjunctival epithelial cells is released into heterophagosomes brought within the lysosomal compartment. The number of secondary phagosomes seems to be increased by intercellular transport of latex spheres to the acid phosphatase rich cells in the deep layers of the epithelium.

Keywords

Acid Phosphatase Acid Phosphatase Activity Autophagic Vacuole Superficial Cell Conjunctival Epithelium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Axline SG, Cohn ZA (1970) In vitro induction of lysosomal enzymes by phagocytosis. J Exp Med 131:1239–1260PubMedCrossRefGoogle Scholar
  2. Brunk UT, Ericsson JL (1972) The demonstration of acid phosphatase in vitro cultured tissue cells. Studies of the significance of fixation, tonicity and permeability. Histochem J 4:349–363PubMedCrossRefGoogle Scholar
  3. Carleton HM (1931) Studies on epithelial phagocytosis. Proc R Soc Biol Sci 108:1–10Google Scholar
  4. Dark AJ, Durrant TE, McGinty F, Shortland JR (1974) Tarsal conjunctiva of the upper eyelid. Am J Ophthalmol 77:555–564PubMedGoogle Scholar
  5. de Duve C, Wattiaux R (1966) Functions of lysosomes. Annu Rev Physiol 28:435–492PubMedCrossRefGoogle Scholar
  6. Glaumann H, Ericsson JLE, Marzella L (1981) Mechanisms of intralysosomal degradation with special reference to autophagocytosis and heterophagocytosis of cell organelles. Int Rev Cytol 73:149–182PubMedCrossRefGoogle Scholar
  7. Hayashi M (1967) Comparative histochemical localisation of lysosomal enzymes in rat tissues. J Histochem Cytochem 15:83–92PubMedGoogle Scholar
  8. Hollyfield JG, Ward A (1974) Phagocytic activity of the pigmented retinal epithelium. III. Interaction between lysosomes and ingested polystyrene spheres. Invest Ophthalmol 13:1016–1023PubMedGoogle Scholar
  9. Hopwood D, Logan KR, Milne G (1978) The light and electron microscopic distribution of acid phosphatase activity in human normal oesophageal epithelium. Histochem J 10:159–170PubMedCrossRefGoogle Scholar
  10. Howard HJ (1924) Role of the epithelial cell in conjunctival and corneal infections. Am J Ophthalmol 7:909–936Google Scholar
  11. Iqbal M, Gerson S (1971) Biochemical features of oral epithelium. In: Squieres CA, Meyer J (eds) Current concepts of the histology of oral mucosa. CC Thomas, Springfield, pp 34–60Google Scholar
  12. Latkovic S, Nilsson SEG (1979a) The ultrastructure of the normal conjunctival epithelium of the guinea pig. I. The basal and intermediate layers of the perilimbal zone. Acta Ophthalmol 57:106–122Google Scholar
  13. Latkovic S, Nilsson SEG (1979b) Phagocytosis of latex microspheres by the epithelial cells of the guinea pig conjunctiva. Acta Ophthalmol 57:582–590Google Scholar
  14. Latkovic S, Nilsson SEG (1984) The phagocytic capability of the various zones of the guinea pig conjunctival epithelium: an electron microscopic study. Acta Ophthalmol 62:453–460CrossRefGoogle Scholar
  15. Lindner K (1921) Über die Topographie der parasitären Bindehautkeime. A v Graefes Arch Klin Ophthalmol 105:726–777Google Scholar
  16. Novikoff AB (1973) Lysosomes: a personal account. In: Hers HG, van Hoof F (eds) Lysosomes and storage diseases. Academic Press, New York London, pp 1–41Google Scholar
  17. Novikoff AB, Novikoff PM (1977) Cytochemical contributions to differentiating GERL from the Golgi apparatus. Histochem J 9:525–551PubMedCrossRefGoogle Scholar
  18. Schnyder J, Baggiolini M (1978) Role of phagocytosis in the activation of macrophages. J Exp Med 148:1449–1457PubMedCrossRefGoogle Scholar
  19. Segal AW, Dorling J, Coade S (1980) Kinetics of fusion of the cytoplasmic granules with phagocytic vacuoles in human polymorphonuclear leukocytes. J Cell Biol 85:42–59PubMedCrossRefGoogle Scholar
  20. Woff K, Konrad K (1972) Phagocytosis of latex beads by epidermal keratinocytes in vivo. J Ultastruct Res 39:262–280CrossRefGoogle Scholar
  21. Wolff K, Schreiner E (1970) Epidermal lysosomes. Electron microscopic — cytochemical studies. Arch Dermatol 101:276–286PubMedCrossRefGoogle Scholar
  22. Zimianski MC, Dawson CR, Togni B (1974) Epithelial cell phagocytosis of Listeria monocytogenes in the conjunctiva. Invest Ophthalmol 13:623–626PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • S. Latkovic
    • 1
  1. 1.Department of OphthalmologyUniversity of LinköpingLinköpingSweden

Personalised recommendations