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Molecular events in the processing of avidin by antigen-presenting cells (APC)

III. Activation of T-lymphocyte lines andH-2 restriction are mediated by processed avidin associated withI-region gene products

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Abstract

We investigated the interaction between processed avidin (PA) and avidin-specific lines of T lymphocytes free of resident antigen-presenting cells (APC). We found that PA was able to replace the requirement for APC in the T-lymphocyte proliferative assay, only if the PA was associated with an Ia-positive moiety (IPM) supplied by the APC. In addition to supplying a necessary signal for a proliferative response to PA, IPM imposedH-2 restriction on the PA molecule. The association between PA and IPM was reversible and the two moieties could be physically separated and recombined. The results support a conclusion that major histocompatibility restriction of the interaction between T lymphocytes and APC is due to the association between processed antigen and an APC element containing I-region products.

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Abbreviations

APC:

antigen-presenting cells

Ir:

genes immune-response genes

PA:

processed avidin

NA:

native avidin

LNC:

lymph-node cells

BSA:

bovine serum albumin

PPD:

purified protein derivative

CS:

control supernatant

PBSA:

processed BSA

IPM:

Ia-positive moiety

References

  • Bayer, E. A. and Wilchek, M.: The use of the avidin biotin complex as a tool in molecular biology.In D. Glick (ed.):Methods of Biochemical Analysis, Vol. 26, pp. 1–45, John Wiley & Sons, New York, 1980

    Google Scholar 

  • Benacerraf, B.: A hypothesis to relate the specificity of T lymphocytes and the activity of I region specific Ir genes in macrophages and B lymphocytes.J. Immunol. 120: 1809–1812, 1978

    Google Scholar 

  • Benacerraf, B. and Germain, R. V.: The immune response genes of the major histocompatibility complex.Immunol. Rev. 38: 70–119, 1978

    Google Scholar 

  • Ben-Nun, A. and Cohen, I. R.: Experimental autoimmune encephalomyelitis (EAE) mediated by T-cell lines: Process of selection of lines and characterization of cells.J. Immunol. 129: 303–309, 1982

    Google Scholar 

  • Clark, R. B. and Shevach, E. M.: Generation of T cell colonies from responder strain 2 guinea pigs that recognize the copolymer L-glutamine acid, L-lysine in association with nonresponder strain 13 la antigens.J. Exp. Med. 155: 635–640, 1982

    Google Scholar 

  • Cohen, I. R. and Talmon, J.: H-2 genetic control of the response of T lymphocytes to insulins. Priming of nonresponder mice by forbidden variants of specific antigenic determinants.Ear. J. Immunol. 10: 284–289, 1980

    Google Scholar 

  • Cohen, I. R., Talmon, J., Lev-Ram, V., and Ben-Nun, A.: Immune response genes have a variable effect on the selection of antigen foreign and self determinants of insulin.Proc. Natl. Acad. Sci. U.S.A. 76: 4066–4070, 1979

    Google Scholar 

  • Erb, P. and Feldman, M.: The role of macrophages in the generation of T helper cells. III. Influence of macrophage derived factors in helper cell induction.Eur. J. Immunol. 5: 759–766, 1975

    Google Scholar 

  • Erb, P., Feldman, M., and Hogg, N.: The role of macrophages in the generation of T helper cells. IV. Nature of genetically related factor derived from macrophages incubated with soluble antigens.Eur. J. Immunol. 6: 365–372, 1976

    Google Scholar 

  • Eshhar, Z., Strassman, G., Waks, T., and Mozes, E.: In vitro and in vivo effector cells mediating DTH responses to a protein and synthetic polypeptide antigen.Cell Immunol. 47: 378–389, 1979

    Google Scholar 

  • Friedman, A. and Cohen, I. R.: Molecular events in the processing of avidin by antigen-presenting cells (APC). I. The immune response of T lymphocytes to avidin is regulated byH-2-LinkedIr genes.Immunogenetics 18: 267–275, 1983

    Google Scholar 

  • Friedman, A., Gitler, C., and Cohen, I. R.: Molecular aspects of the processing and presentation of avidin by macrophages to T lymphocytes.In C. W. Pirece, S. E. Cullen, J. A. Kapp, B. D. Schwartz, and D. C. Shreffler (eds.):Ir Genes: Past and Present, pp. 284–293, Humana Press, Clifton, N. J., 1983a

    Google Scholar 

  • Friedman, A., Zerubavel, R., Gitler, C., and Cohen, I. R.: Molecular events in the processing of avidin by antigen-presenting cells (APC). II. Identical processing by APC ofH-2 high- and low-responder mouse strains.Immunogenetics 18: 277–289, 1983b

    Google Scholar 

  • Germain, R. H.: Accessory cell stimulation of T cell proliferation requires active antigen processing, la restricted antigen presentation and a separate nonspecific 2nd signal.J. Immunol. 127: 1964–1966, 1981

    Google Scholar 

  • Green, M.:Avidin. Adv. Protein Chem. 29: 85–133, 1975

    Google Scholar 

  • Grossman, Z. and Conen, I. R.: A theoretical analysis of the phenotypical expression of immune response genes.Ear. J. Immunol. 10: 633–640, 1980

    Google Scholar 

  • Howie, S. and McBride, W. H.: Cellular interactions in thymus dependent antibody responses.Immunol. Today 3: 273–278, 1982

    Google Scholar 

  • Ishii, N., Baxevanis, C. N., Nagy, Z. A., and Klein, J.: Responder T cells depleted of alloreactive cells react to antigen presented on allogeneic macrophages from nonresponder strains.J. Exp. Med. 154: 978–982, 1981

    Google Scholar 

  • Lee, K-C., Singh, B., Barton, M. A., Procyshyn, A., and Wong, M.: A simple reliable system for studying antigen specific murine T cell proliferation.J. Immunol. Methods 25: 159–170, 1979

    Google Scholar 

  • Lonai, P., Steinman, L., Friedman, V., Drizlikh, G., and Puri, J.: Specificity of antigen binding by T cells. Competition between soluble and la-associated antigen.Eur. J. Immunol. 11: 382–387, 1981

    Google Scholar 

  • Matzinger, P.: A one-receptor view of T cell behaviour.Nature 292: 497–501, 1981

    Google Scholar 

  • Nagy, Z. A. and Klein, J.: Macrophage or T cell, that is the question.Immunol. Today 2: 288–299, 1981

    Google Scholar 

  • Puri, J. and Lonai, P.: Mechanism of antigen binding by T cells. H-2 (I-A-restricted binding of antigen plus la by helper cells.Eur. J. Immunol. 10: 273–281, 1980

    Google Scholar 

  • Ranghino, G., Talmon, J., Yonath, A., and Cohen, I. R.: The conformation of antigenic determinants of insulin and H-2 gene control of the immune response of T lymphocytes.In M. Balaban, J. L. Sussman, W. Traub, and A. Yonath (eds.):Structural Aspects of Recognition and Assembly in Biological Macromolecules, pp. 263–279, Balaban ISS, Rehovot, Philadelphia, 1981

    Google Scholar 

  • Rosenthal, A. S.: Determinant selection and macrophage function in genetic control of immune responses.Immunol. Rev. 40: 135–152, 1978

    Google Scholar 

  • Rosenthal, A. S.: Determinant selection and macrophage function.Immunol. Today 3: 33–34, 1982

    Google Scholar 

  • Schwartz, R. H.: A clonal deletion model for Ir gene control of the immune response.Scand. J. Immunol. 7: 3–10, 1978

    Google Scholar 

  • Schwartz, R. A., Yano, A., and Paul, W. E.: Interaction between antigen presenting cells and primed T lymphocytes. An assessment for Ir gene expression in the antigen presenting cell.Immunol. Rev. 40: 153–180, 1978

    Google Scholar 

  • Schwartz, R. A., Yano, A., Stimpfling, J. H., and Paul, W. E.: Gene complementation in the T lymphocyte proliferative response to GLO: A demonstration that both immune response genes must be expressed in the same antigen presenting cells.J. Exp. Med. 149: 40–57, 1979

    Google Scholar 

  • Sercarz, E. E., Yowell, R. L., Turkin, D., Miller, A., Araneo, B. A., and Adorini, L.: Different functional specificity repertoires for suppressor and helper T cells.Immunol. Rev. 39: 108–136, 1978

    Google Scholar 

  • Shevach, E.: Is determinant selection dead?Immunol. Today 3: 31–33, 1982

    Google Scholar 

  • Skutelsky, E. and Bayer, E. A.: The ultrastructural localization of cell surface glycoconjugates. Affinity cytochemistry via the avidin biotin complex.Biol. Cell. 36: 237–252, 1979

    Google Scholar 

  • Snedcore, G. W. and Cochran, W. G.:Statistical Methods, The Iowa State University Press, Ames, Iowa, 1967

    Google Scholar 

  • Todd, R. F., Reinharz, E. L., and Schlossman, S. F.: Human macrophagelymphocyte interaction in proliferation to soluble antigen. I. Specific deletion of lymphocyte proliferative activity on macrophage monolayers.Cell Immunol. 55: 114–121, 1980

    Google Scholar 

  • Unanue, E. R.: The regulatory role of macrophages to antigen stimulation, Part 2. Symbiotic relationship between lymphocytes and macrophages.Adv. Immunol. 31: 1–135, 1981

    Google Scholar 

  • Yano, A., Schwartz, R. H., and Paul, W. E.: Antigen presentation in the murine T lymphocyte proliferative response. I. Requirement for genetic identity of the major histocompatibility complex.J. Exp. Med. 146: 828–843, 1977

    Google Scholar 

  • Ziegler, H. K. and Unanue, E. R.: Identification of a macrophage antigen processing event required for I region restricted antigen presentation to T lymphocytes.J. Immunol. 127: 1869–1875, 1981

    Google Scholar 

  • Ziegler, H. K. and Unanue, E. R.: Decrease in macrophage antigen catabolism caused by ammonia and chloroquinine is associated with inhibition of antigen presentation to T cells.Proc. Natl. Acad. Sci. U.S.A. 79: 175–170, 1982

    Google Scholar 

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Authors and Affiliations

  1. Department of Cell Biology, The Weizmann Institute of Science, P. O. Box 26, 76100, Rehovot, Israel

    Aharon Friedman, Rachel Zerubavel & Irun R. Cohen

  2. Department of Membrane Research, The Weizmann Institute of Science, P. O. Box 26, 76100, Rehovot, Israel

    Carlos Gitler

Authors
  1. Aharon Friedman
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  2. Rachel Zerubavel
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  3. Carlos Gitler
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  4. Irun R. Cohen
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Friedman, A., Zerubavel, R., Gitler, C. et al. Molecular events in the processing of avidin by antigen-presenting cells (APC). Immunogenetics 18, 291–302 (1983). https://doi.org/10.1007/BF00952967

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  • DOI: https://doi.org/10.1007/BF00952967

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