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Molecular events in the processing of avidin by antigen-presenting cells (APC)

I. The immune response of T lymphocytes to avidin is regulated byH-2-linkedIr genes

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Abstract

The immune response of T lymphocytes to avidin was measured by proliferative assays, antibody production and delayed-type hypersensitivity. Mice ofH-2 k haplotypes were found to be low responders, whereas mice of other haplotypes, and particularly ofH-2 s, were high responders.Ir genes controlling this response were mapped to theI subregion ofH-2. Helper T cells were found to be responsible for the Ir phenotype of antibody production. These results indicate the feasibility of using the avidin-biotin complex as a tool for studying molecular mechanisms by which antigens underIr gene control are processed and presented to T lymphocytes.

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Abbreviations

Ir :

genes, immune-response genes

H-2 :

murine major histocompatibility complex

APC:

antigen-presenting cell

OA:

ovalbumin

BSA:

bovine serum albumin

DNP:

dinitrophenyl

DNP-OA:

DNP-ovalbumin

DNP-Av:

DNP-avidin

DNP-BSA:

DNP-bovine serum albumin

CFA:

complete Freund's adjuvant

PPD:

purified protein derivative

PBS:

phosphatebuffered saline

IP:

intraperitoneal

LNC:

lymph-node cells

DTH:

delayed-type hypersensitivity

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Authors and Affiliations

  1. Department of Cell Biology, The Weizmann Institute of Science, 76100, Rehovot, Israel

    Aharon Friedman & Irun R. Cohen

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  1. Aharon Friedman
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  2. Irun R. Cohen
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Friedman, A., Cohen, I.R. Molecular events in the processing of avidin by antigen-presenting cells (APC). Immunogenetics 18, 267–275 (1983). https://doi.org/10.1007/BF00952965

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  • DOI: https://doi.org/10.1007/BF00952965

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