Some sources of error in the calculation of relative change in plasma volume

  • M. H. Harrison
  • M. J. Graveney
  • Lynda A. Cochrane


The effects of alterations in the F-cell ratio (i.e., the ratio of the whole body haematocrit to the peripheral haematocrit) on calculations of relative (percentage) change in plasma volume have been considered. Errors introduced by failing to correct the measured haematocrit for an assumed value for the F-cell ratio are small, and are negligible compared with the random measurement errors. Errors introduced by a change in the value of the F-cell ratio from some control value to a new value during an experimental procedure (such as exercise or exposure to heat stress) are large, and could lead to gross misrepresentations of the direction and magnitude of any induced change in intravascular volume, and of solute concentration changes assumed to reflect only loss or gain of fluid by the intravascular space.

Key words

Plasma volume Haematocrit Haemoglobin F-cell ratio 


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  1. Albert SN, Gravel V, Turmel Y, Albert CA (1965) Pitfalls in blood volume measurement. Anesthesia and Analgesia. Curr Res 44: 805–814Google Scholar
  2. åstrand P-O, Saltin B (1964) Plasma and red cell volume after prolonged severe exercise. J Appl Physiol 19: 829–832Google Scholar
  3. Beaumont W van, Greenleaf JE, Juhos L (1972) Disproportional changes in haematocrit, plasma volume, and proteins during exercise and bed rest. J Appl Physiol. 33: 55–61Google Scholar
  4. Beaumont W van, Underkofler W, Beaumont S van (1981) Erythrocyte volume, plasma volume, and acid-base changes in exercise and heat dehydration. J Appl Physiol: Respirat Environ Exercise Physiol 50: 1255–1262Google Scholar
  5. Chaplin H, Mollison PL (1953) Correction for plasma trapped in the red cell column of the hematocrit. Blood 7: 1227–1238Google Scholar
  6. Chaplin H, Mollison PL, Vetter H (1953) The body/venous hematocrit ratio: its constancy over a wide hematocrit range. J Clin Invest 32: 1309–1316Google Scholar
  7. Cochrane LA, Edwards RJ, Graveney MJ, Harrison MH (1981) Effects of alterations in skin temperature on intravascular volume and protein content in resting subjects. RAF Institute of Aviation Medicine Report No 595Google Scholar
  8. Convertino VA, Greenleaf JE, Bernauer EM (1980) Role of thermal and exercise factors in the mechanism of hypervolemia. J Appl Physiol: Respirat Environ Exercise Physiol 48: 657–664Google Scholar
  9. Costill DL, Saltin B (1974) Changes in the ratio of venous to body hematocrit following dehydration. J Appl Physiol 36: 608–610Google Scholar
  10. Dill DB, Costill DL (1974) Calculation of percentage changes in volumes of blood, plasma and red cells in dehydration. J Appl Physiol 37: 247–248Google Scholar
  11. Ebert RV, Stead EA (1941) Demonstration that in normal man no reserves of blood are mobilised by exercise, epinephrine and haemorrhage. Am J Med Sci 201: 655–664Google Scholar
  12. Elkinton JR, Danowski TS, Winkler AW (1945) Hemodynamic changes in salt depletion and in dehydration. J Clin Invest 25: 120–129Google Scholar
  13. Fricke G von (1965) über das Verhalten den Zellfaktors bei körperlicher Arbeit. Cardiologia 47: 25–44Google Scholar
  14. Garby L, Vuille JG (1961) The amount of trapped plasma in a high speed micro-capillary hematocrit centrifuge. J Clin Lab Invest 13: 642–645Google Scholar
  15. Gibson JG, Seligman AM, Peacock WC, Aub JC, Fine J, Evans RD (1946) The distribution of red cells and plasma in large and minute vessels of the normal dog, determined by radioactive isotopes of iron and iodine. J Clin Invest 25: 848–857Google Scholar
  16. Greenleaf JE, Convertino VA, Stremel RW, Bernauer EM, Adams WC, Vignau SR, Brock PJ (1977) Plasma [Na+], [Ca+2], and volume shifts and thermoregulation during exercise in man. J Appl Physiol: Respirat Environ Exercise Physiol 43: 1026–1032Google Scholar
  17. Greenleaf JE, Convertino VA, Mangseth GR (1979) Plasma volume during stress in man: osmolality and red cell volume. J Appl Physiol: Respirat Environ Exercise Physiol 47: 1031–1038Google Scholar
  18. Gregerson MI, Rawson RA (1959) Blood volume. Physiol Rev 39: 307–342Google Scholar
  19. Harrison MH (1974) Plasma volume changes during acute exposure to high environmental temperature. J Appl Physiol 37: 38–42Google Scholar
  20. Harrison MH, Edwards RJ, Leitch DR (1975) Effects of exercise and thermal stress on plasma volume. J Appl Physiol 39: 925–931Google Scholar
  21. Harrison MH, Edwards RJ, Graveney MJ, Cochrane LA, Davies JA (1981) Blood volume and plasma protein responses to heat acclimatisation in humans. J Appl Physiol: Respirat Environ Exercise Physiol 50: 597–604Google Scholar
  22. Kaltreider NL, Meneely GR (1940) The effect of exercise on the volume of the blood. J Clin Invest 19: 627–634Google Scholar
  23. Nylin G (1947) The effect of heavy muscular work on the volume of circulating red corpuscles in man. Am J Physiol 149: 180–184Google Scholar
  24. Pugh LGCE (1969) Blood volume changes in outdoor exercise of 8–10 hour duration. J Physiol 200: 345–351Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • M. H. Harrison
    • 1
  • M. J. Graveney
    • 1
  • Lynda A. Cochrane
    • 1
  1. 1.R. A. F. Institute of Aviation MedicineFarnboroughEngland

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