International Ophthalmology

, Volume 18, Issue 4, pp 211–214 | Cite as

Corneal autofluorescence in diabetic and normal eyes

  • Sergio Fantaguzzi
  • Franco Docchio
  • Lauretta Guarisco
  • Rosario Brancato


Corneal autofluorescence has been lately studied as a predictor of retinopathy severity in diabetic patients. We measured corneal autofluorescence in 138 eyes of 69 diabetic patients and 64 eyes of 32 healthy controls. Diabetic patients were subdivided by the severity of retinopathy according to the Modified Airlie House Classification (stage 1: no or minimal retinopathy; stage 2: minimal background retinopathy; stage 3: background retinopathy; stage 4: (pre-) proliferative retinopathy. The fluorescence peak value and the area underlying the corneal autofluorescence curve were measured with a scanning fluorophotometer (Fluorotron Master, Coherent Radiation Palo Alto CA) Healthy controls' values of corneal autofluorescence (peak value: mean 11.03±3.77 ng. equivalent fluorescein/ml; area: mean 163.85±61.40 scan-point × ng. equivalent fluorescein/ml) resulted similar (peak value: p=0.83; area: p=0.61) to those of diabetic patients without retinopathy (peak value: mean 11.2±3.4 ng.eq/ml; area: 170.07±57.23 A statistically significant difference was found between diabetic patients without retinopathy and patients with stage 2, 3, 4 retinopathy. No statistically significant difference was found both for the peak value (p=0.50) and for the area (p=0.61) between stage 3 and stage 4 retinopathy. The sensitivity and specificity of corneal autofluorescence as a screening test for diabetic retinopathy were 82% and 62% for the peak value, 87% and 60% for the area; the positive predictive value for the presence of diabetic retinopathy was 65% for the peak and 63% for the area.

Key words

corneal autofluorescence diabetes fluorophotometry retinopathy grade 


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  1. 1.
    Schwartz DE. Corneal sensitivity in diabetics. Arch Ophthalmol 1974; 91: 174–8.Google Scholar
  2. 2.
    Busted N, Olsen T, Schmitz O. Clinical observations on the corneal thickness and the corneal endothelium in diabetes mellitus. Br J Ophthalmol 1981; 65: 687–90.Google Scholar
  3. 3.
    Schultz RO, van Horn DL, Peters MA, Klewin KM, Schutten WH. Diabetic keratopathy. Trans Am Ophthalmol 1981; 79: 180–99.Google Scholar
  4. 4.
    Bleeker JC, van Best J, Vrij L, van der Velde EA, Oosterhuis JA. Autofluorescence of the lens in diabetic and healthy subjects by fluorophotometry. Invest Ophthalmol Vis Sci 1986; 27: 791–4.Google Scholar
  5. 5.
    Occhipinti JR, Mosier MA, Burstein NL. Autofluorescence and light transmission in the aging crystalline lens. Ophthalmologica 1986; 192:203–9.Google Scholar
  6. 6.
    Van Best J, van Gessel P. Autofluorescence and light scatter in the human lens as measured by a fluorophotometer. Exp Eye Res 1989; 49: 511–3.Google Scholar
  7. 7.
    Larsen M, Kjer B, Bendtson I, Dalgaard P, Lund-Andersen H. Lens fluorescence in relation to metabolic control of insulin-dependent diabetes mellitus. Arch Ophthalmol 1989; 107: 59–62.Google Scholar
  8. 8.
    Stolwijk TR, van Best J, Boot JP, Oosterhuis JA. Corneal autofluorescence in diabetic and penetrating keratoplasty patients as measured by fluorophotometry. Exp Eye Res 1990;51:403–9.Google Scholar
  9. 9.
    Stolwijk TR, van Best J, Oosterhuis JA, Swart W. Corneal autofluorescence: an indicator of diabetic retinopathy. Invest Ophthalmol Vis Sci 1982; 33: 92–7.Google Scholar
  10. 10.
    Diabetic Retinopathy Study Research Group: Report 7: A modification of the Airlie House classification of diabetic retinopathy. Invest Ophthalmol Vis Sci 1981; 21: 210–6.Google Scholar
  11. 11.
    Van Best J, Kappelhof JP, Laterveer L, Oosterhuis JA. Blood aqueous barrier permeability versus age by fluorophotometry. Curr Eye Res 1987; 6: 855–63.Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Sergio Fantaguzzi
    • 1
  • Franco Docchio
    • 2
  • Lauretta Guarisco
    • 1
  • Rosario Brancato
    • 1
  1. 1.Department of OphthalmologyUniversity of Milano, Scientific Institute H-SRaffaeleItaly
  2. 2.Dipartimento di Elettronica per l'AutomazioneUniversity of BresciaItaly

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