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Planta

, Volume 161, Issue 1, pp 1–11 | Cite as

Periclinal penetration of potassium permanganate into mature cuticular membranes ofAgave andClivia leaves: new implications for plant cuticle development

  • Joachim Wattendorff
  • Peter J. Holloway
Article

Abstract

Staining cuticular membranes ofAgave americana andClivia miniata en bloc with potassium permanganate results in a strong contrast in the interior cuticular layer while the exterior part remains unstained. This is not caused by a selective chemical reaction with the interior part but by the unidirectional penetration of the reagent from the interior side, the outside being protected by the cuticle proper. In transverse cryosections of the cuticular membrane, permanganate penetrates nearly as easily into the exterior cuticular layer as into the interior one giving the same contrast. However, compared with the periclinal penetration into the cuticle proper this penetration is accelerated five-to tenfold by the polysaccharide network within the cuticular layer which serves as a distribution-channel system. Periclinal penetration into the cuticle proper occurs independently in each “cutin penetration unit” included between two obvious lucent lamellae and further divided into subunits.

Key words

Agave Clivia Cuticle development Cuticular membrane (ultrastructure) Cutin (polar) Cutin synthesis Periclinal penetration Polysaccharide network Potassium penetration Wax 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Joachim Wattendorff
    • 1
    • 2
  • Peter J. Holloway
    • 1
    • 2
  1. 1.Institut für Botanische Biologie der UniversitätFreiburgSwitzerland
  2. 2.Long Ashton Research StationUniversity of BristolBristolUK

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