Diagnosis of intraocular lymphoma by polymerase chain reaction

  • Naomichi Katai
  • Sachiko Kuroiwa
  • Kazuya Fujimori
  • Nagahisa Yoshimura
Clinical Investigation


• Background: Cytopathological examinations have been used in the diagnosis of intraocular lymphoma. However, sometimes it is not easy to detect malignant cells in the biopsy specimens. We applied a method that identified monoclonal proliferation of B lymphocytes by using polymerase chain reaction (PCR) in the diagnosis of patients suspected to have intraocular B-cell lymphoma. • Methods: Threè specimens of the diagnostic vitrectomy were studied by cytological examination and by PCR to amplify the complementary determining region (CDR3) of immunoglobulin heavy chain (IgH) gene. As a positive control, a biopsy specimen of an orbital lymphoma was examined; four vitrectomy specimens from patients with diabetic retinopathy, proliferative vitreo-retinopathy, acute retinal necrosis (ARN) and macular hole were negative controls. • Results: On cytologic examination, no malignant cells were found in three specimens: suspected intraocular lymphoma and one ARN. In contrast, a discrete band that reflected monoclonal proliferation of B lymphocytes was detected by PCR in specimens from two patients and the positive control. Vitrectomy specimens from the negative controls, including ARN, did not show a discrete band on PCR. • Conclusions: Two cases of ocular malignant lymphoma were diagnosed by PCR identification of monoclonal proliferation of B lymphocytes. This method may be an additional diagnostic tool in the diagnosis of intraocular B-cell lymphoma.


Polymerase Chain Reaction Lymphoma Diabetic Retinopathy Malignant Lymphoma Macular Hole 
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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Naomichi Katai
    • 1
  • Sachiko Kuroiwa
    • 1
  • Kazuya Fujimori
    • 2
  • Nagahisa Yoshimura
    • 1
  1. 1.Department of OphthalmologyShinshu University School of MedicineMatsumotoJapan
  2. 2.Second Department of Internal MedicineShinshu University School of MedicineMatsumotoJapan

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