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Intracorneal bovine albumin: an immunologic model of corneal angiogenesis

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Abstract

• Background: We characterized the neovascularization that follows the intracorneal injection of bovine albumin (BA) in rabbits as a model of corneal angiogenesis. • Methods: New Zealand white rabbits received intracorneal injections of phosphate-buffered saline with and without various amounts of BA. The rabbits were co-sensitized or presensitized by intramuscular BA or were not sensitized. The corneal vascular response was quantified by ranking photographs taken periodically after the injection. • Results: In pre-sensitized animals, blood vessels were apparent within 4 days and reached maximum intensity 14 days after the intracorneal injection. Corneas also vascularized in nonsensitized rabbits, but a larger dose (>0.2 mg BA) was required than in pre-sensitized animals (>0.02 mg BA). Vascularization began later in non-sensitized animals and was less extensive than in pre-sensitized animals. • Conclusion: The intracorneal injection of BA is a reproducible model of corneal angiogenesis in rabbits and should allow the involved immunological mechanisms to be elucidated.

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Authors and Affiliations

  1. Medizinische Hochschule, Hanover, Germany

    Thomas Damms

  2. Duke University Eye Center, P.O. Box 3802, 27710, Durham, NC, USA

    Joel R. Ross, Michael D. Duplessie & Gordon K. Klintworth

Authors
  1. Thomas Damms
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  2. Joel R. Ross
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  3. Michael D. Duplessie
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  4. Gordon K. Klintworth
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Correspondence to Gordon K. Klintworth.

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Damms, T., Ross, J.R., Duplessie, M.D. et al. Intracorneal bovine albumin: an immunologic model of corneal angiogenesis. Graefe's Arch Clin Exp Ophthalmol 235, 662–666 (1997). https://doi.org/10.1007/BF00946944

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  • DOI: https://doi.org/10.1007/BF00946944

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