Influence of global ischemia on the sarcolemmal ATPases in the rat heart
To elucidate the effect of global ischemia on the energy utilizing processes, regarding the molecular principles, the kinetic and thermodynamic properties of the sarcolemmal ATPases were investigated in the rat heart. The activation energy for hydrolysis of ATP during ischemia was higher when the reaction was catalyzed by Ca-ATPase or Mg-ATPase. For the Na,K-ATPase reaction, no changes in the activation energy were observed. With respect to the enzyme kinetics, ischemia in a timedependent manner induced important alterations in KM and Vmax values of Na,K-ATPase, Ca-ATPase and Mg-ATPase. The Vmax value decreased significantly already after 15 min of ischemia, and it also remained low after 30, 45 and 60 min for all 3 enzymes. The significant diminution of KM values occurred later in the 30th min for Ca-ATPase, in the 45th min for Na,K-ATPase. The observed drop in KM indicates the increase in the affinity of the enzymes to substrate, suggesting thus the adaptation to ischemic conditions on the molecular level. This effect could be attributed to some conformational changes of the protein molecule in the vicinity of the ATP-binding site developing after longer duration of ischemia.
Key wordscardiac sarcolemma (Na,K)-ATPase Mg-ATPase Ca-ATPase enzyme kinetics activation energy ischemia
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