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A macrolide antibiotic, roxithromycin, inhibits the growth of human myeloid leukemia HL60 cells by producing multinucleate cells

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Abstract

The antiproliferative effect of roxithromycin (RXM) was studied using human myeloid leukemia HL60 cells. RXM inhibited the growth of HL60 cells in a concentration-dependent manner, and significantly inhibited growth at concentrations above 75 μM. This growth inhibition was not associated with specific cell cycle arrest and DNA synthesis was not impaired. In addition, the number of viable cells remained almost unchanged in the presence of 100 νM RXM. RXM induced growth inhibition at least partly by the formation of multinucleate cells. Both flowcytometric and morphological examination revealed that more than 40% of the RXM-treated cells were binucleate. These findings demonstrate that RXM is a potent new modulator of cell cycle progression in HL60 cells and suggest that the inhibition of cytokinesis by this drug may provide a new model for studying mitosis.

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Authors and Affiliations

  1. Department of Internal medicine, Aoto Hospital, The Jikei University School of Medicine, 6-41-2 Aoto, Katsushika-ku, Tokyo 125, Japan

    Makoto Nagai, Hisashi Yamada, Shuji Nakada, Keiko Ochi, Tadashi Nemoto, Shinobu Takahara & Junko Horiguchi-Yamada

  2. Department of Clinical Laboratory, The Jikei University School of Medicine, 6-41-2 Aoto, Katsushika-ku, Tokyo 125, Japan

    Sadayori Hoshina

Authors
  1. Makoto Nagai
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  2. Hisashi Yamada
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  3. Shuji Nakada
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  4. Keiko Ochi
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  5. Tadashi Nemoto
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  6. Shinobu Takahara
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  7. Sadayori Hoshina
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  8. Junko Horiguchi-Yamada
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Nagai, M., Yamada, H., Nakada, S. et al. A macrolide antibiotic, roxithromycin, inhibits the growth of human myeloid leukemia HL60 cells by producing multinucleate cells. Mol Cell Biochem 144, 191–195 (1995). https://doi.org/10.1007/BF00944399

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  • DOI: https://doi.org/10.1007/BF00944399

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