Abstract
Polyclonal antibodies to mouse α- and β/γ-caseins were raised in rabbits. These antibodies display tissue- and species specificity as shown by immunoblotting. Immunohistochemical analyses demonstrate that both α- and β/γ-caseins were synthesized and secreted from virtually all lactating mammary epithelial cells, in a pattern very similar to that of the mouse α-lactalbumin. Residual amounts of caseins were located also in the apical surface of epithelial cells surrounding the ducal lumen of virgin mammary gland sections. In contrast to the significant level of γ-casein in the milk, the amount of this protein compared to α- or β-caseins was extremely low in medium conditioned for 24 h by mammary explants of mid-pregnant mice immediately after explantation or after 4 days.
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Barash, I., Faerman, A., Puzis, R. et al. Synthesis and secretion of caseins by the mouse mammary gland: Production and characterization of new polyclonal antibodies. Mol Cell Biochem 144, 175–180 (1995). https://doi.org/10.1007/BF00944397
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DOI: https://doi.org/10.1007/BF00944397