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Cloning and functional expression of the bovine natriuretic peptide receptor-B (natriuretic factor R1c subtype)

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Abstract

We describe the isolation of a 3,276 base pair cDNA for the bovine natriuretic peptide receptor-B (NPR-B). Expression of this clone in Cos-P cells demonstrates that it encodes an agonist-dependent guanylyl cyclase. Porcine CNP stimulates the activity of this receptor up to 200-fold with an ED50 of 12±2 nM, whereas brain natriuretic peptide C-type natriuretic peptide (CNP) and atrial natriuretic factor (ANF) are less efficacious. In addition, ligand binding studies indicate that this receptor exhibits the pharmacology appropriate for the bovine NPR-B. CNP binds to Cos-P cell membranes expressing this clone with a Kd of 13±1 pM, and natriuretic peptides compete for [125I]-CNP binding with a rank order of pCNP>pBNP>rANF. Thus, the expressed receptor-guanylyl cyclase exhibits the expected pharmacological profile for ligand binding and cyclase activation of the bovine NPR-B receptor.

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Abbreviations

BSA:

bovine serum albumin

dNTP:

deoxynucleotide triphosphate

SDS:

sodium dodecyl sulfate

DEAE-dextran:

diethylaminoethyl-dextran

EDTA:

ethylenediamine tetraacetic acid

Tris:

Tris(hydroxymethyl)aminomethane

DMSO:

dimethyl sulfoxide

RP-HPLC:

reverse phase-high performance liquid chromatography

AMV:

avian myeloblastosis virus

Arg:

arginine

Lys:

lysine

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Authors and Affiliations

  1. Department of Pharmacology, University of Montreal, Station “Centre-ville”, P.O. Box 6128, H3C 3J7, Montreal, Quebec, Canada

    Normand McNicoll & André De Léan

  2. Department of Biochemistry, University of Montreal, H3C 3J7, Montreal, Quebec, Canada

    Randy Fenrick, Marc Therrien & Jacques Drouin

  3. Faculty of Pharmacy, University of Montreal, H3C 3J7, Montreal, Quebec, Canada

    Kasimir Babinski

  4. Clinical Research Institute of Montreal, Canada

    Marc Therrien & Jacques Drouin

Authors
  1. Randy Fenrick
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  2. Kasimir Babinski
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  3. Normand McNicoll
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  4. Marc Therrien
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  5. Jacques Drouin
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  6. André De Léan
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Fenrick, R., Babinski, K., McNicoll, N. et al. Cloning and functional expression of the bovine natriuretic peptide receptor-B (natriuretic factor R1c subtype). Mol Cell Biochem 137, 173–182 (1994). https://doi.org/10.1007/BF00944079

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  • DOI: https://doi.org/10.1007/BF00944079

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