Theoretical and Experimental Chemistry

, Volume 26, Issue 1, pp 98–101 | Cite as

Solvation effects in human serum albumin radiolysis in the presence of dimethyl sulfoxide

  • V. K. Pogorelyi
  • V. N. Barvinchenko
  • V. V. Turov
Brief Communications


PMR and electrophoresis have been applied to examine hydration changes in protein molecules due to the presence of the electron donor dimethylsulfoxide DMSO, which influences the radiolysis of human serum albumin HSA. The reactions of aqueous HSA with added DMSO show that the DMSO on the one hand acts as a protector, which prevents the formation of low-molecular protein forms on reaction with hydroxyl radicals, and on the other alters the protein hydration, which facilitates thiol-di-sulfide exchange, which leads to oligomers.


Hydration Albumin Hydroxyl DMSO Electrophoresis 
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Literature cited

  1. 1.
    N. A. Vysotskaya, A. F. Rekasheva, and L. G. Shevchuk, Reaction Treatment for Effluent Sediments [in Russian], Tekhnika, Kiev (1978).Google Scholar
  2. 2.
    N. A. Vusotskaya, L. G. Shevchuk, I. I. Kukhtenko, et al., “Research on the mechanism whereby ionizing radiation improves water loss from deposits,” Khim. Tekhnol. Vody,3, No. 5, 413–418 (1981).Google Scholar
  3. 3.
    V. K. Pogorelyi, V. V. Turov, A. V. Turov, and V. N. Barvinchenko, “PMR spectra of frozen aqueous solutions of serum albumin and the interactions with dimethyl sulfoxide,” Teor. Eksp. Khim.,25, No. 1, 104–108 (1989).Google Scholar
  4. 4.
    K. Weber and M. Osborn, “The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis,” J. Biol. Chem.,244, No. 16, 4406–4412 (1969).Google Scholar
  5. 5.
    A. K. Pikaev and S. A. Kabakchi, Reactivities of Primary Water Radiolysis Products [in Russian], Énergoizdat, Moscow (1982).Google Scholar
  6. 6.
    M. Joly, Protein Denaturation Physical Chemistry [Russian translation], Mir, Moscow (1968).Google Scholar
  7. 7.
    Yu. M. Torchinskii, Sulfur in Proteins [in Russian], Nauka, Moscow (1977).Google Scholar
  8. 8.
    V. A. Sharpatyi, Biopolymer Radiation Chemistry [in Russian], Énergoizdat, Moscow (1981).Google Scholar
  9. 9.
    I. P. Gragerov, V. K. Pogorelyi, and I. F. Franchuk, Hydrogen Bonds and Fast Proton Exchange [in Russian], Naukova Dumka, Kiev (1978).Google Scholar

Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • V. K. Pogorelyi
    • 1
  • V. N. Barvinchenko
    • 1
  • V. V. Turov
    • 1
  1. 1.Pisarzhevskii Physical Chemistry InstituteUkrainian Academy of SciencesKiev

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