Abstract
The distribution of phosphatidylethanolamine in the two lipid layers of chromatophores ofRhodospirillum rubrum has been analysed by chemical modification of phosphatidylethanolamine (PE) with trinitrobenzenesulfonic acid (TNBA) at low temperatures. Around 45±1% of the total phosphatidylethanolamine is labelled by this procedure independent on chromatophore purity, vesicle size, action of proteases and growth state of the cells. This demonstrates a complete modification of the accessible phosphatidylethanolamine and an asymmetric distribution of phosphatidylethanolamine, with 45% of the phosphatidylethanolamine in the outer part of the bilayer.
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Abbreviations
- TNBA:
-
2,4,6 trinitrobenzenesulfonic acid
- PE:
-
phosphatidylethanolamine
- PMS:
-
phenazinmethosulfate
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DaPra, E., Snozzi, M. & Bachofen, R. Distribution of phosphatidylethanolamine in the lipid bilayer of chromatophores of the photosynthetic bacterium rhodospirillum rubrum. Arch. Microbiol. 133, 23–27 (1982). https://doi.org/10.1007/BF00943764
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DOI: https://doi.org/10.1007/BF00943764