Removal of RNA by heat treatment
- 153 Downloads
Eight bacterial strains were subjected to a discontinuous heat shock treatment aimed at causing a degradation of RNA. The treatment involved a 10 s to 10 min exposure to 65°C and then an incubation period of up to 3 h at 50°C. At intervals the cells were analyzed for RNA, DNA and protein. Whereas the contents of protein and DNA were not affected, RNA was degraded. An almost complete degradation of RNA occurred inAlcaligenes eutrophus H 16 — PHB−4 andEscherichia coli K 12; only about 50% of the cellular RNA were degraded inPseudomonas putida andP.flava GA; inCorynebacterium autotrophicum 7 C,Nocardia opaca 1 b and coryneform strains 11 X and 30.1 b RNA degradation occurred only to a small extent.
A continuous flow system for the treatment of cell suspensions by heat shock followed by incubation at an elevated temperature was developed. The results confirmed those obtained by batch-wise heat treatment.
KeywordsHeat Treatment Cell Suspension Heat Shock Elevated Temperature Bacterial Strain
Unable to display preview. Download preview PDF.
- Abu Ruwaida, A.S., Lafferty, R.M., Schlegel, H.G. (1976). Europ. J. Appl. Microbiol.2, 73–79Google Scholar
- Auling, G. (1975). Intakte und defekte Phagen von Wasserstoffbakterien, Doctoral thesis, GöttingenGoogle Scholar
- Baumgarten, J., Reh, M., Schlegel, H.G. (1974). Arch. Microbiol.100, 207–217Google Scholar
- Herbert, D., Phipps, P.S., Strange, R.E. (1971). Chemical analysis of microbial cells. In: Methods in microbiology, J.R. Norris, D.W. Ribbons, eds., vol. 5B, New York, London: Academic PressGoogle Scholar
- Maul, S.B., Sinskey, A.J., Tannenbaum, S.R. (1970). Nature229, 181Google Scholar
- Pfennig N., Lippert, K.D. (1966). Arch. Mikrobiol.55, 245–256Google Scholar
- Tunail, N., Schlegel, H.G. (1974). Arch. Microbiol.100, 341–350Google Scholar