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A monoclonal antibody raised against adultSchistosoma mansoni which recognizes a surface antigen on schistosomula

Zeitschrift für Parasitenkunde volume 70, pages 189–197 (1984)Cite this article

Abstract

A monoclonal antibody has been raised by immunizing a mouse with an isolated tegumental preparation of adultSchistosoma mansoni. The hybridoma, designated NIMP/M.47, secreted an IgG2a antibody which was positive by indirect immunofluorescence with live schistosomula ofS. mansoni, but not with live schistosomula ofS. bovis, or with other living life cycle stages ofS. mansoni. In complement-dependent, or cell-mediated in vitro cytotoxicity assays, the monoclonal antibody mediated levels of schistosomular killing as high as those obtained with sera from infected mice. No significant protection, however, was obtained in passive transfer experiments. NIMP/M.47 was specific for a 20,000 dalton polypeptide in the schistosomular surface, which was also recognized by serum from infected mice.

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Author notes

  1. C. A. P. Tavares

    Present address: ICB-UFMG, 30000, Belo Horizonte, Brazil

Affiliations

  1. Division of Parasitology, National Institute for Medical Research, Mill Hill, NW7 IAA, London, UK

    C. A. P. Tavares, R. de Rossi, G. Payares, A. J. G. Simpson, D. J. McLaren & S. R. Smithers

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  1. C. A. P. Tavares
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  2. R. de Rossi
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  3. G. Payares
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  4. A. J. G. Simpson
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  5. D. J. McLaren
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  6. S. R. Smithers
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Tavares, C.A.P., de Rossi, R., Payares, G. et al. A monoclonal antibody raised against adultSchistosoma mansoni which recognizes a surface antigen on schistosomula. Z. Parasitenkd. 70, 189–197 (1984). https://doi.org/10.1007/BF00942221

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  • DOI: https://doi.org/10.1007/BF00942221

Keywords

  • Monoclonal Antibody
  • Life Cycle
  • Polypeptide
  • Surface Antigen
  • Cytotoxicity Assay