Zeitschrift für Parasitenkunde

, Volume 66, Issue 1, pp 17–24 | Cite as

Stimulating effect of citrate and cis-aconitate on the transformation ofTrypanosoma brucei bloodstream forms to procyclic forms in vitro

  • R. Brun
  • M. Schönenberger
Original Investigations


Transformation ofTrypanosoma brucei bloodstream forms to procyclic forms can be stimulated in vitro by adding 2–10 mM citrate and/or cis-aconitate to the culture medium for at least 3 h. Optimal transformation was obtained in the presence of 3 mM cis-aconitate for 48 h. Citrate and cis-aconitate may activate isocitrate dehydrogenase and/or other Krebs' cycle enzymes and thus act as a metabolic trigger for the morphological changes involved in transformation.


Citrate Morphological Change Isocitrate Isocitrate Dehydrogenase Cycle Enzyme 
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  1. Barrett J (1978) Activation of succinate dehydrogenase from adultFasciola hepatica (Trematoda). Parasitology 76:269–275PubMedGoogle Scholar
  2. Bienen EJ, Hammadi E, Hill GC (1981)Trypanosoma brucei: Biochemical and morphological changes during in vitro transformation of bloodstream- to procyclic trypomastigotes. Exp Parasitol 51:408–417PubMedGoogle Scholar
  3. Bowman IBR, Srivastava HK, Flynn IW (1972) Adaptions in oxidative metabolism during the transformation ofTrypanosoma rhodesiense from bloodstream into culture form. In: H Van den Bossche (ed) Comparative biochemistry of parasites. Academic Press, New York London, pp 329–342Google Scholar
  4. Bowman IBR, Flynn IW (1976) Oxidative metabolism of trypanosomes. In Lumsden WHR, Evans DA (eds) Biology of the Kinetoplastida. Academic Press, New York London, pp 435–476Google Scholar
  5. Brown RC, Evans DA, Vickerman K (1973) Changes in oxidative metabolism and ultrastructure accompanying differentiation of the mitochondrion inTrypanosoma brucei. Int J Parasitol 3:691–704PubMedGoogle Scholar
  6. Brun R, Schönenberger M (1979) Cultivation and in vitro cloning of procyclic culture forms ofTrypanosoma brucei in a semi-defined medium. Acta Trop (Basel) 36:289–292Google Scholar
  7. Cunningham I (1977) New culture medium for maintenance of tsetse tissue and growth of Trypanosomatids. J Protozool 24:325–329PubMedGoogle Scholar
  8. Flynn IW, Bowman IBR (1973) The metabolism of carbohydrate by pleomorphic African trypanosomes. Comp Biochem Physiol [B] 45:25–42Google Scholar
  9. Ghiotto V, Brun R, Jenni L, Hecker H (1979)Trypanosoma brucei: Morphometric changes and loss of infectivity during transformation of bloodstream forms to procyclic culture forms in vitro. Exp Parasitol 48:447–456PubMedGoogle Scholar
  10. Hanson RS, Srinivasan VR, Halvorson HO (1963) Biochemistry of sporulation. II. Enzymatic changes during sporulation ofBacillus cereus. J Bacteriol 86:45–50PubMedGoogle Scholar
  11. Hill GC (1976) Characterization of the electron transport systems present during the life cycle of African trypanosomes. In: H van den Bossche (ed) Biochemistry of parasites and host-parasite relationships Elsevier/North-Holland Biomedical Press, Amsterdam, pp 31–50Google Scholar
  12. Ryley JF (1962) Studies on the metabolism of the protozoa. 9. Comparative metabolism of bloodstream and culture forms ofTrypanosoma rhodesiense. Biochem J 85:211–223PubMedGoogle Scholar
  13. Wyatt GR (1961) The biochemistry of insect haemolymph. Ann Rev Entomol 6:75–102Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • R. Brun
    • 1
  • M. Schönenberger
    • 1
  1. 1.Swiss Tropical InstituteBaselSwitzerland

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