Abstract
Destruction of the basement membrane (BM) is mandatory for tumor spread, and matrix metalloproteinases MMPs) are known to be implicated in colon cancer invasion and metastasis by digesting type IV collagen, a main component of the BM. The current study analyzed the expression of MMP-2 and MMP-9 in pancreatic cancer tissues. Frozen specimens of pancreatic cancer (n = 10), a liver metastatic nodule from pancreatic cancer (n = 1), and normal pancreas (n = 3) were homogenized and analyzed by zymography. The activated form of MMP-9 (82kDa) was detected in all of the normal and malignant tissues, while the activated form of MMP-2 (62kDa) was detected in all of the pancreatic cancers and its metastatic tissue, but not in the normal pancreatic tissues. These results indicate that expression of the activated form of MMP-2 may be specific to pancreatic cancer, while that of MMP-9 may be unrelated to it.
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This study was supported in part by a grant (no. 07457271) from the Japanese Ministry of Education
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Koshiba, T., Hosotani, R., Wada, M. et al. Detection of matrix metalloproteinase activity in human pancreatic cancer. Surg Today 27, 302–304 (1997). https://doi.org/10.1007/BF00941802
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DOI: https://doi.org/10.1007/BF00941802