Effects of mitomycin C on the expression of proliferating cell nuclear antigen after filtering surgery in rabbits

  • Joji Watanabe
  • Shoichi Sawaguchi
  • Takeo Fukuchi
  • Haruki Abe
  • LiLi Zhou
Laboratory Investigation


• Background: The aim of the study was to evaluate the effects of mitomycin C on the cell replication activity in wound healing following experimental filtration surgery. • Methods: Trabeculectomy with or without application of mitomycin C was performed on albino rabbit eyes. At 1, 4, 7, 14, and 28 days after surgery, the expression of proliferating cell nuclear antigen, a marker of cell proliferation, in the filtering site was examined immunohistochemically using a streptavidin-biotin complex method. • Results: In control eyes that underwent trabeculectomy but did not receive mitomycin C, the number of immunoreactive cells increased 4–7 days after operation and decreased markedly at around 14 days. The filtering site was obstructed histologically at 4–7 days after operation. In the mitomycin C-treated eyes, immunoreactive cells appeared 4 days after surgery but disappeared by 7 days at the surgical site. The number of immunoreactive cells in the treated eyes was much lower than that in control eyes. • Conclusion: The cell replication activity was markedly inhibited by administration of mitomycin C. The filtering site remained open for 28 days after surgery, whereas it was completely obstructed within 7 days in control eyes. Immunocytochemistry for proliferating cell nuclear antigen, as used in this study, is a simple and reliable method for detection of cell replication activity.


Public Health Filtration Wound Healing Reliable Method Proliferate Cell Nuclear Antigen 
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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Joji Watanabe
    • 1
  • Shoichi Sawaguchi
    • 1
  • Takeo Fukuchi
    • 1
  • Haruki Abe
    • 1
  • LiLi Zhou
    • 2
  1. 1.Department of Ophthalmology, Niigata UniversitySchool of MedicineNiigataJapan
  2. 2.Department of Ophthalmology and Visual SciencesUniversity of Illinois at Chicago College of MedicineChicagoUSA

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