Cytochemical localization of guanylate cyclase in photoreceptor cells of the mouse
- 28 Downloads
• Background: Light-stimulated excitation causes a decrease of the cGMP concentration in vertebrate photoreceptor cells. The cGMP content is restored by the catalytic action of a guanylate cyclase (EC 220.127.116.11). • Methods: The spatial distribution of guanylate cyclase was determined cytochemically in rod visual cells of the mouse. • Results: In retinal tissue of the mouse guanylate cyclase was found throughout the photoreceptor cells, in the outer and the inner segments, and was especially prominent in the cilia and in elongations of cilia extending into the outer segments. A reaction product of adenylate cyclase (EC 18.104.22.168) could not be demonstrated in vertebrate rod outer segments. • Conclusion: The relatively high amount of guanylate cyclase in the inner segments and the cilia may contribute — at least in part — to the actual concentration and the time course of concentration changes of the cGMP concentration in rod outer segments.
KeywordsPublic Health Spatial Distribution Actual Concentration Catalytic Action Adenylate Cyclase
Unable to display preview. Download preview PDF.
- 11.Rack M, Rubly N, Waschow C (1986) Effects of some chemical reagents on sodium current inactivation in myelinated nerve fibers of the frog. Biophys 150:557–564Google Scholar
- 12.Saito T (1977) Histochemical methods using heavy metals. Acta Histochem Cytochem 10:226–29Google Scholar
- 13.Saito T, Araki M, Akahoshi T (1980) Lead citrate as a capture reagent for demonstration of adenylate and guanylate cyclase activities. In: Lake BD, High OB, Holt SJ, Stoward PJ (eds) VIth International Histochemistry and Cytochemistry Congress 1980. Royal Microscopical Society, Oxford, p 335Google Scholar
- 15.Toibana M, Tsukahara I, Ogawa K (1982) Cytochemical demonstration of guanylate cyclase activity in retinal photoreceptors with special reference to changes under light and dark adaptation. Acta Histochem Cytochem 15:5–20Google Scholar