Abstract
The tandemly repeated multigene families encoding 18S and 25S rRNAs were studied at the restriction enzyme level inPopulus alba L.,Populus deltoides Bartr. exMarsh.,Populus trichocarpa Torr. & Gray and in the hybrids between the last two mentioned species. The analysis of single and double digestion with EcoRI, BamHI, XbaI, and SstI endonucleases showed the presence of single repetitive unit types of 12.25 and 11.75kb inP. alba andP. trichocarpa, respectively.P. deltoides showed two rDNA gene types having the same length (12.25Kb) but different nucleotide sequence in the IGS. The rDNAs genes ofP. deltoides andP. triochocarpa are inherited codominantly in their hybrids.
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References
Appels, R., Dvorak, J., 1982a: The wheat ribosomal DNA spacer region: its structure and variation in populations and among species. — Theor. Appl. Genet.63: 337–348.
—, —, 1982b: Relative rates of divergence of spacer and gene sequences within the rDNA region of species in theTriticeae: implications for the maintenance of homogeneity of a repeated gene family. — Theor. Appl. Genet.63: 361–365.
Bellarosa, R., Del Re, V., Schirone, B., Maggini, F., 1990: Ribosomal RNA genes inQuercus spp. (Fagaceae). — Pl. Syst. Evol.172: 127–139.
Carmona, M. J., De Dominicis, R. I., Salvi, G., Maggini, F., 1984: Ribosomal RNA genes in biotypes ofScilla peruviana (Liliaceae). — Pl. Syst. Evol.146: 1–11.
Dickmann, D. I., Stuart, K. W., 1983: The culture of poplars in eastern north America. — Michigan State University.
Dvorak, J., McGuire, P. E., Cassidy, B., 1988: Apparent sources of the A genomes of wheats inferred from polymorphism in abundance and restriction fragment length of repeated nucleotide sequences. — Genome30: 680–689.
Erickson, J., Rushford, C., Dorney, D., Wilson, G., Schmickel, R., 1981: Structure and variation of human ribosomal DNA: molecular analysis of cloned fragments. — Gene16: 1–9.
Ganal, M., Hemleben, V., 1986: Comparison of the ribosomal RNA genes in four closely relatedCucurbitaceae. — Pl. Syst. Evol.154: 63–77.
Gerlach, W. L., Bedbrook, J. R., 1979: Cloning and characterization of ribosomal RNA genes from wheat and barley. — Nucleic Acids Res.7: 1869–1885.
Heilman, P. E., Stettler, R. F., 1985: Genetic variation and production ofPopulus trichocarpa T. & G. and its hybrids. 2. Biomass production in a 4-year plantation. — Canad. J. Forest Res.15: 384–388.
Kiss, T., Kis, M., Solymosy, F., 1989a: Nucleotide sequence of a 25 S rRNA gene from tomato. — Nucleic Acids Res.17: 796.
—,Szkukalek, A., Solymosy, F., 1989b: Nucleotide sequence of a 17S (18S) rRNA gene from tomato. — Nucleic Acids Res.17: 2127.
Lassner, M., Dvorak, J., 1986: Preferential homogenization between adjacent and alternate subrepeats in wheat rDNA. — Nucleic Acids Res.14: 5499–5512.
Long, E. O., Dawid, I. B., 1980: Repeated genes in eukaryotes. — Annu. Rev. Biochem.49: 727–764.
Maggini, F., Carmona, M. J., 1981: Sequence heterogeneity of the ribosomal DNA inAllium cepa (Liliaceae). — Protoplasma108: 163–171.
—,Tucci, G., Gelati, M. T., 1988: Ribosomal RNA genes in species of theCynareae tribe (Compositae). 2. — Protoplasma144: 125–131.
May, C. E., Appels, R., 1987: Variability and genetic of spacer DNA sequences between the ribosomal-RNA genes of hexaploid wheat (Triticum aestivum). — Theor. Appl. Genet.74: 617–624.
Molnar, S. J., Gupta, P. K., Fedak, G., Wheatcroft, R., 1989: Ribosomal DNA repeat unit polymorphism in 25Hordeum species. — Theor. Appl. Genet.78: 387–392.
Pace, N. R., Olsen, G. J., Woese, C. R., 1986: Ribosomal RNA phylogeny and the primary lines of evolutionary descent. — Cell45: 325–326.
Polans, N. O., Weeden, N. F., Thompson, W. F., 1986: Distribution, inheritance and linkage relationships of ribosomal DNA spacer length variants in pea. — Theor. Appl. Genet.72: 289–295.
Rogers, S. O., Bendich, A. J., 1987: Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. — Pl. Molec. Biol.9: 509–520.
—,Honda, S., Bendich, A. J., 1986: Variation in the ribosomal RNA genes among individuals ofVicia faba. — Pl. Molec Biol.6: 339–345.
Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A., Allard, R. W., 1984: Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. — Proc. Natl. Acad. Sci. U.S.A.81: 8014–8018.
Southern, E., 1975: Detection of specific sequences among DNA fragments separated by gel electrophoresis. — J. Molec. Biol.98: 503–509.
Wahl, G. M., Stern, M., Stark, G. R., 1979: Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethal-paper and rapid hybridization by using dextran sulfate. — Proc. Natl. Acad. Sci. U.S.A.76: 3683–3685.
Yakura, K., Kato, A., Tanifuji, S., 1984: Length heterogeneity in the large spacer ofVicia faba rDNA is due to the differing number of a 325 bp repetitive sequence elements. — Molec. Gen. Genet.193: 400–405.
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D'Ovidio, R., Mugnozza, G.S. & Tanzarella, O.A. Ribosomal RNA genes structure in somePopulus spp. (Salicaceae) and their hybrids. Pl Syst Evol 173, 187–196 (1990). https://doi.org/10.1007/BF00940862
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DOI: https://doi.org/10.1007/BF00940862