Abstract
The cultivars or experimental lines ofPisum sativum were analyzed cytophotometrically for nuclear DNA content of early prophases after ethanol-acetic acid fixation. Wide variability was found (from 3.93 to 5.07pg per haploid nucleus). This result was confirmed by the cytophotometric analysis of interphase nuclei isolated from leaf tissues fixed in formalin. Analysis of interphase nuclei at different thresholds of optical density showed that certain chromatin fractions are involved in the variations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bennett, M. D., 1972: Nuclear DNA content and minimum generation time in herbaceous plants. — Proc. Royal Soc. London, ser. B,181: 109–135.
—, 1987: Variation in genomic form in plants and its ecological implications. — New Phytol.106: 177–200.
—,Smith, J. B., 1976: Nuclear DNA amounts in angiosperms. — Philos. Trans. Roy. Soc. London, ser. B274: 227–274.
Cavallini, A., Natali, L., 1989: Cytological analyses of in vitro somatic embryogenesis inBrimeura amethystina Salisb. (Liliaceae) — Pl. Sci.62: 255–261.
—,Cionini, P. G., D'Amato, F., 1981: Location ofHeitz's Zerstäubungsstadium (dispersion phase) in the mitotic cycle ofPhaseolus coccineus and the concept of angiosperm endomitosis. — Protoplasma109: 403–414.
—,Zolfino, C., Cionini, G., Cremonini, R., Natali, L., Sassoli, O., Cionini, P. G., 1986: Nuclear DNA changes withinHelianthus annuus L.: cytophotometric, karyological and biochemical analyses. — Theor. Appl. Genet.73: 20–26.
Cionini P. G., 1989: Nuclear DNA changes during plant development — Nuovo G. Bot. Ital. (in press).
Cullis, C. A., Davies, D. R., 1975: Ribosomal DNA amounts inPisum sativum. — Genetics81: 485–492.
D'Amato, F., 1985: Cytogenetics of plant cell and tissues cultures and their regenerates. — CRC Crit. Rev. Pl. Sci.3: 73–112.
Deumling, B., Clermont, L., 1989: Changes in DNA content and chromosomal size during cell culture and plant regeneration ofScilla siberica: selective chromatin diminution in response to environmental conditions. — Chromosoma97: 439–448.
Ellis, T. H. N., Davies, D. R., Castleton, J. A., Bedford, I. D., 1984: The organization and genetics of rDNA length variants in peas. — Chromosoma91: 74–81.
Evans, L. S., Van't Hof, J., 1975: Is polyploidy necessary for tissue differentiation in higher plants? — Amer. J. Bot.62: 1060–1064.
Frölich, E., Nagl, W., 1979: Transitory increase in chromosomal DNA (Feulgen) during floral differentiation inRhoeo discolor. — Cell Diff.8: 11–18.
Greilhuber, J., 1986: Severely distorted Feulgen-DNA amounts inPinus (Coniferophytina) after non-additive fixations as a result of meristematic self-tanning with vacuole contents. — Canad. J. Genet. Cytol.28: 409–415.
—, 1988: ”Self-tanning“—a new and important source of stoichiometric error in cytophotometric determination of nuclear DNA content in plants. — Pl. Syst. Evol.158: 87–96.
Levi, M., Tarquini, F., Sgorbati, S., Sparvoli, E., 1986: Determination of DNA content by static cytofluorimetry in nuclei released from fixed plant tissue. — Protoplasma132: 64–68.
Lewis, W. H., 1980: Polyploidy in species populations. — InLewis, W. H., (Ed.): Polyploidy, biological relevance, pp. 103–144. — New York, London: Plenum.
Mello, M. L. S., Vidal, B. C., 1980: Acid lability of deoxyribonucleic acids of some polytene chromosome regions ofRhynchosciara americana. — Chromosoma81: 419–429.
Nagl, W., 1979: Differential DNA replication in plants: a critical review. — Z. Pflanzenphysiol.95: 283–314.
Price, H. J., 1988: Nuclear DNA content variation within angiosperm species. — Evol. Trends Plants2: 53–60.
—,Bachmann, K., Chambers, K. L., Riggs, J., 1980: Detection of intraspecific variation in nuclear DNA content inMicroseris douglasii. — Bot. Gaz.141: 195–198.
Schweizer, D., Davies, D. R., 1972: Nuclear DNA contents ofPisum grown in vivo and in vitro. — Planta106: 23–29.
Walbot, V., Cullis, C. A., 1985: Rapid genome changes in higher plants. — Annu. Rev. Pl. Physiol.36: 367–396.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cavallini, A., Natali, L. Nuclear DNA variability withinPisum sativum (Leguminosae): Cytophotometric analyses. Pl Syst Evol 173, 179–185 (1990). https://doi.org/10.1007/BF00940861
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00940861