Advertisement

Ethanol production from sugarcane bagasse holocellulose. Coupled systems for saccharification, xylose isomerization and yeast fermentation

  • Raquel L. Salomón
  • Carlos M. Cuevas
  • Guillermo R. Ellenrieder
Research Papers Presented at the First Latin American Congress on Biotechnology, Tucumán, Argentina (October 1987)

Summary

Ethanol production from sugarcane bagasse holocellulose. Coupled systems for saccharification, xylose isomerization and yeast fermentation.

An isomerization step was added to the coupled system for ethanol production from sugar cane bagasse in order to allow the alcoholic fermentation of xylose as xylulose bySaccharomyces cerevisiae. The yield (g ethanol/g pretreated bagasse) was improved according to the method of pretreatment: 0.185 to 0.24 with NaOH pretreatment, 0.11 to 0.18 for Ca(OH)2 and 0.26 to 0.29 for a combined Ca(OH)2/NaCl method.

Keywords

Fermentation Xylose Cane Sugarcane Ethanol Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Résumé

L'ajout d'une étape d'isomérisation à un système couplé pour la production d'éthanol à partir de bagasse de cane à sucre a permis la fermentation éthanolique tant du xylose que du xylulose parSaccharomyces cerevisiae. Le rendement en g d'éthanol par g de bagasse prétraitée a crû selon la méthode de prétraitement: de 0.185 à 0.24 en cas de prétraitement à la soude caustique, de 0.11 à 0.18 dans le cas de Ca(OH)2 et de 0.26 à 0.29 dans le cas de la méthode combinée Ca(OH)2-NaCl.

Resumen

Se estudio la inculsión de una etapa de isomerización en el sistema de columnas acopladas, de producción de etanol a partir del bagazo de caña de azúcar, con el fin de permitir asi la fermentación alcohólica de la xilosa. El rendimiento expresado como g etanol/g bagazo pretratado, aumentó dependiendo del tipo de pretratamiento: 0.185 a 0.24 para el pretratamiento con NaOH; 0.11 a 0.18 para el correspondiente a Ca(OH)2 y 0.26 a 0.29 para un tratamiento combinado de Ca(OH)2-NaCl.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alurralde, J. L. andEllenrieder, G. (1984). Effect of attached carbohydrates on the activity ofTrichoderma viride cellulases.Enzyme and Microbial Technology 6, 467–470Google Scholar
  2. Blanco, S., Gamarra, A., Cuevas, C. &Ellenrieder, G. (1982). Ethanol production by coupled saccharification and fermentation of sugar cane bagasse.Biotechnology Letters.4, 661–666.Google Scholar
  3. Blanco, S., Gamarra, A., Cuevas, C. &Ellenrieder, G. (1984). Ethanol production by coupled saccharification and fermentation of sugar cane bagasse (II).Memorias del Tercer Simposio Panamericano de Combustibles y Productos Quimicos Via Fermentación. Avances en la Producción de Etanol. pp. 92–103. ICAITI, Guatemala.Google Scholar
  4. Chen, W. P. (1980). Glucose Isomerase.Process Biochemistry, 30–39.Google Scholar
  5. Chiang, L. C. Hsiao, H. Y., Ueng, P. P., Chen, L. F. &Tsao, G. T. (1981). Ethanol production from xylose by enzymic isomerization and yeast fermentation.Biotechnology and Bioengineering Symposium 11, 263–274.Google Scholar
  6. David, C., Fornasier, R. &Thiry, Ph. (1985) Utilization of waste cellulose. VI Pretreatment of lignocellulosic materials with sodium hypochlorite and enzymatic hydrolysis byTrichoderma viride.Applied Biochemistry and Biotechnology 11, 351–365.Google Scholar
  7. Dekker, R. F. H. (1985). Biodegradation of the hemicelluloses. InBiosynthesis and degradation of wood components Higuchi T., 505–553 London, Academic Press.Google Scholar
  8. Ellenrieder, G. &Castillo, J. J. (1983). Evaluation of pretreatments favouring enzymatic hydrolysis of sugar cane bagasse.Latin American Journal of Chemical Engineering and Applied Chemistry 13, 199–214.Google Scholar
  9. Fox, D. J., Gray, P. P., Dunn, N. W. &Marsden, W. L. (1984). An explanation of the discrepancy between the results of HPLC and DNS assays in the analysis of the lignocellulosic hydrolysates.Journal of Chemical Technology and Biotechnology 34B, 171.Google Scholar
  10. Gong, C. S., Chen, L. F., Flickinger, M. C., Chiang, L. C. &Tsao, G. T. (1981) Production of ethanol fromd-xylose by usingd-xylose isomerase and yeast.Applied and Environmental Microbiology 41, 430–436.Google Scholar
  11. Hahn-Haegerdahl, B., Berner, S. &Koog, S. (1986). Improved ethanol production from xylose with glucose isomerase andS. cerevisiae.Applied Microbiology and Biotechnology 24, 287–293.Google Scholar
  12. Hsiao, H. Y., Chiang, L. C., Chen, L. F. &Tsao, G. (1982). Effect of borate on isomerization and yeast fermentation of high xylulose solution and acid hydrolysate of hemicellulose.Enzyme and Microbial Technology 4, 25–31.Google Scholar
  13. IUPAC. (1987). Measurements of cellulase activities.Pure and Applied Chemistry 59 (2), 257–268.Google Scholar
  14. Jeffries, T. W., (1981). Fermentation of xylose to ethanol using xylose isomerase and yeast.Biotechnology and Bioengineering Symposium 11, 315–324.Google Scholar
  15. Mandels, M., Andreotti, R. &Roche, C. (1976). Measurement of saccharifying cellulase.Biotechnology and Bioengineering Symposium 6, 21–33.Google Scholar
  16. Mes-Hartree, M., Hogan, C., Hayes, R. D. &Saddler, J. N. (1983) Enzymatic hydrolysis of agricultural residues byTrichoderma cellulases and the fermentation of liberated sugars to ethanol.Biotechnology Letters 5, 101–110.Google Scholar
  17. Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar.Analytical Chemistry 31 426–428.Google Scholar
  18. Poutanen, K., Puls, J. &Linko, M. (1987). The hemicellulolytic activities ofTrichoderma reesei.Proceedings of the 4th European Congress of Biotechnology.Vol.2, p. 90. Amsterdam. Elsevier.Google Scholar
  19. Wang, P. Y., Johnson, B. F. &Schneider, H. (1980). Fermentation ofd-xylose by yeast using glucose isomerase in the medium to convertd-xylose tod-xylulose.Biotechnology Letters 2, 273.Google Scholar
  20. Williams, D. &Munnecke, E. D. M. (1981). The production of ethanol by immobilized yeast cells.Biotechnology and Bioengineering 23, 1813–1825.Google Scholar
  21. Yamanaka, K. (1966).d-Xylose isomerase.Methods in Enzymology 9, 589–593.Google Scholar

Copyright information

© Oxford University Press 1988

Authors and Affiliations

  • Raquel L. Salomón
    • 1
  • Carlos M. Cuevas
    • 1
  • Guillermo R. Ellenrieder
    • 1
  1. 1.Instituto de Investigaciones para la Industria Quimica (INIQUI)Universidad nacional de SaltaSaltaRepública Argentina

Personalised recommendations