Applied Microbiology and Biotechnology

, Volume 29, Issue 2–3, pp 258–263 | Cite as

Overproduction and excretion ofβ-lactamase and alkaline phosphatase byEscherichia coli olp mutants

  • Brigitte Magnouloux-Blanc
  • Raymond Portalier
Applied Genetics and Regulation

Summary

We have isolated spontaneousolp mutants ofEscherichia coli K-12 overproducing the periplasmic enzymes β-lactamase (Bla) and alkaline phosphatase (PhoA). Enzyme overproduction was maintained inolp strains transformed with plasmids carryingbla+ andphoA+ structural genes, and synthesizing high levels of Bla and PhoA. Transformedolp strains excreted up to 40% of these enzymes into the growth medium. The introduction of atolA excretory mutation intoolp strains led to an increase of enzyme overproduction and a release of 85% of Bla and PhoA enzyme activities into the culture medium.

Keywords

Enzyme Enzyme Activity Alkaline Phosphatase Growth Medium Structural Gene 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Brigitte Magnouloux-Blanc
    • 1
  • Raymond Portalier
    • 1
  1. 1.Atelier de Biotechnologie Enzymatique et Microbienne du Centre National de la Recherche Scientifique (UM 380024)Université Claude Bernard, Lyon IVilleurbanne cedexFrance

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