Applied Microbiology and Biotechnology

, Volume 41, Issue 4, pp 440–446 | Cite as

Development of a transformation system forTrichoderma longibrachiatum and its use for constructing multicopy transformants for theegl1 gene

  • P. Sánchez-Torres
  • R. González
  • J. A. Pérez-González
  • L. González-Candelas
  • D. Ramón
Applied Genetics and Regulation Original Paper


An efficient transformation system for the fungusTrichoderma longibrachiatum has been developed. Transformation was obtained both by electroporation and polyethyleneglycol treatment, using a plasmid carrying theEscherichia coli hygromycin B phosphotransferase gene as a dominant selectable marker. The transformation frequency was 0.5 to 5 transformants /μg plasmid DNA. Transformation normally occurred by tandem integration of the transforming DNA. A high percentage of the transformants were mitotically unstable. The efficiency of co-transformation was very high (around 90%), and several co-transformants containing multiple copies of theegll gene encoding a β-(1,4)-endoglucanase were obtained. Some of them secrete increased levels of endoglucanase to the culture medium. In addition, theE. coli lacZ gene was expressed in an active form under control of theAspergillus nidulans gpdA gene promoter.


Active Form Gene Promoter Hygromycin Efficient Transformation Multiple Copy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • P. Sánchez-Torres
    • 1
  • R. González
    • 1
  • J. A. Pérez-González
    • 1
  • L. González-Candelas
    • 1
  • D. Ramón
    • 1
  1. 1.Unidad de BioingenieríaInstituto de Agroquímica y Tecnología de los Alimentos (C.S.I.C.)Valencia

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