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Applied Microbiology and Biotechnology

, Volume 25, Issue 2, pp 137–142 | Cite as

Isolation and cultivation of a novel microorganism producing a maltopentaose-forming enzyme

  • Hisashi Okemoto
  • Shoichi Kobayashi
  • Mitsuru Momma
  • Hitoshi Hashimoto
  • Kozo Hara
  • Keiji Kainuma
Applied Microbiology

Summary

A novel microorganism producing a maltopentaose-forming enzyme was screened from a soil sample. The enzyme produced by the bacteria formed maltopentaose from starch during the first stage of the reaction. The bacterium was characterized asPseudomonas sp. (KO-8940) on the basis of its morphological, physiological and biological properties. Culture conditions for enzyme production were investigated and established. The medium was composed of beef extract 0.8%, ammonium sulphate 1.0% and maltose 0.8% in tap water. Optimum conditions for bacterial growth were: initial pH 8.0, temperature 45°C, and 3 days cultivation with a rotary shaker (200 rpm). The broth supernatant obtained contained the maltopentaose-forming crude enzyme, with which 40% of starch was converted to maltopentaose.

Keywords

Enzyme Sulphate Ammonium Starch Soil Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Hisashi Okemoto
    • 1
  • Shoichi Kobayashi
    • 2
  • Mitsuru Momma
    • 2
  • Hitoshi Hashimoto
    • 1
  • Kozo Hara
    • 1
  • Keiji Kainuma
    • 2
  1. 1.Ensuiko Sugar Refining Co., Ltd.YokohamaJapan
  2. 2.National Food Research InstituteMinistry of Agriculture, Forestry and FisheriesTsukuba, IbarakiJapan

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