Sensorized on line determinations of molecular hydrogen inEscherichia coli fermentations
The production of molecular hydrogen was measured in the effluent gas of 7 fermentations. The aim of this primary investigation was to study the use of a H2-sensitive metal-oxide-semiconductor structure in physiological studies ofEscherichia coli. In order to yield more information, we measured metabolic heat with a flow microcalorimeter in parallel with the determinations of molecular hydrogen. The physiological parameters of intrest were medial effects, HCOOH-additions, pH and partial oxygen pressure.
KeywordsOxygen Hydrogen Fermentation Partial Oxygen Pressure Medial Effect
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- Cleland N, Hörnsten EG, Elwing H, Enfors S-O, Lundström I (1984) Measurement of hydrogen evolution by oxygen-limitedEscherichia coli by means of a hydrogen sensitive PD-MOS sensor. Appl Microbiol Biotechnol 20:268–270Google Scholar
- Danielsson B, Mattiasson B, Mosbach K (1981) Enzyme thermistor devices and their analytical applications. Appl Biochem Bioeng 3:97–143Google Scholar
- Gest H (1954) Oxidation and evolution of molecular hydrogen by microorganisms. Bact Rev 18:43–73Google Scholar
- Hörnsten EG, Elwing H, Kihlström E, Lundström I (1985a) Sensorized determination of molecular hydrogen in investigations of the susceptibility of Enterobacteriaceae to ampicillin. J Antimicrob Chemother 15:695–700Google Scholar
- Hörnsten EG, Danielsson B, Nilsson L, Elwing H, Lundström I (1985b) Physiological Studies of anEscherichia coli strain under ampicillin stress, anaerobic conditions and spheroplast formation. J Antimicrob Chemother (in press)Google Scholar
- Kondratieva EN, Gogotov IN (1983) Production of molecular hydrogen in microorganisms. Advances in Biochemical Engineering/Biotechnology 28:139–191Google Scholar
- Krab K, Oltmann LF, Stouthammer A (1982) Reduction of oxygen by hydrogen in cells of anaerobically grown Proteus mirabilis. Biochem Biophys Acta 682:59–66Google Scholar
- Lundström I (1981) Hydrogen sensitive MOS-structures Part 1. Principles and Applications. Sensors Actuators 1:403–426Google Scholar
- Schlegel HG, Schneider K (1978). In: Goltze E (ed) Hydrogenases; Their Catalytic Activity, Structure and Function. GöttingenGoogle Scholar
- Winquist F, Spetz A, Lundström I (1984) Determination of ammonia in air and aqueous samples with a gas-sensitive semiconductor capacitor. Analytica Chimica Acta 164:127–130Google Scholar