Abstract
Ribosomal DNA genes fromP. deltoides have been cloned and specific sequences of the 25 S and 18 S rDNA region, labelled by digoxigenin, have been used to determine the rDNA structure ofPopulus tremula, P. fremontii, P. maximowiczii, P. yunnanensis, P. nigra, P. wislizenii, P. alba. The restriction maps of the coding region appeared to be similar among the examined species and with those ofP. deltoides andP. trichocarpa, reported in a previous paper. Inter- and intraspecific variation in rDNA repeat unit length have been revealed after EcoRI digestions. SstI and XbaI restriction sites have been found at different positions in the IGS of some species. The polymorphic fragments generated by SstI digestion allowed the identification of the hybrid origin of some genotypes. The number of rDNA genes in the genome ofP. deltoides has been estimated to be about 2 000 copies. Finally, the usefulness of these studies inPopulus spp. taxonomy and forestry genetics is discussed.
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Ribosomal RNA gene structure in somePopulus spp. (Salicaceae) and their hybrids 2.
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D'Ovidio, R., Mugnozza, G.S. & Tanzarella, O.A. rDNA cloning and rapid hybrid identification inPopulus spp. (Salicaceae). Pl Syst Evol 177, 165–174 (1991). https://doi.org/10.1007/BF00937954
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DOI: https://doi.org/10.1007/BF00937954