Plant Systematics and Evolution

, Volume 166, Issue 1–2, pp 131–139 | Cite as

Isozymes as molecular markers for diploid and tetraploid individuals ofNajas marina (Najadaceae)

  • Ludwig Triest
  • Yzjö Viinikka
  • Moshe Agami


The genetic variability of five natural populations ofNajas marina L., i.e. one diploid of subsp.marina (Europe), two of subsp.intermedia (Europe) and both a diploid (C. Africa) and a tetraploid (Middle East) of subsp.armata, has been estimated by means of electrophoretic studies. These populations differ in their morphology and karyotype. Emphasis is placed on the characteristics and status of a tetraploid cytotype from Merkaz Sappir (Israel). Almost all the variation observed is expressed in seed alcohol dehydrogenase (ADH). The differences are in a unique allele of theAdh-2 locus and in the formation of novel heteromeric isozymes.Adh genes in seeds can be used as a marker for the autotetraploid character. The other enzyme systems tested failed in this respect. The genetic variability based on 23 loci is rather low. Nevertheless, the autotetraploid population has a higher or equal ratio of polymorphic loci than the related diploids. Cluster analysis illustrated not only thatNajas marina subsp.marina has diverged much from subsp.intermedia and subsp.armata, but also showed the difference between the latter two taxa, as well as the intermediate position of the autotetraploid population.

Key words

Angiosperms Najadaceae Najas marina Isozymes polyploidy Polymorphism 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Ludwig Triest
    • 1
  • Yzjö Viinikka
    • 2
  • Moshe Agami
    • 3
  1. 1.Laboratorium voor Algemene Plantkunde en NatuurbeheerVrije Universiteit BrusselBrusselsBelgium
  2. 2.Department of BiologyUniversity of TurkuTurkuFinland
  3. 3.Department of BotanyThe George S. Wise Faculty of Life Sciences, Tel Aviv UniversityTel AvivIsrael

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