Plant Systematics and Evolution

, Volume 188, Issue 3–4, pp 197–211 | Cite as

Chloroplast DNA-based phylogeny of AsianPinus species (Pinaceae)

  • Xiao-Ru Wang
  • Alfred E. Szmidt


The genusPinus includes over 90 species with approximately 24 species native to Asia. We have analyzed the chloroplast (cp) DNA variation of 18Pinus species, including 15 Asian, two Eurasian, and one European species using seven restriction enzymes and ten non-overlapping probes and inferred their phylogenetic relationships. Results of phenetic and cladistic approaches to phylogeny reconstruction were largely in agreement, suggesting two major lineages within the genus and confirmed the ancient character of haploxylon and diploxylon subgenera. Species from sectionParrya appear to have diverged earliest from the hypothesized phylogenetic centre for the haploxylon pines, withP. bungeana andP. gerardiana forming two basal, monotypic lineages. The range of estimated pairwise nucleotide substitutions per site (\(\mathop d\limits^ \sim \)) was higher among haploxylon pines than among diploxylon species. CpDNA divergence was found to be low within the sectionSylvestres, relative to the divergence among haploxylon species, suggesting that the radiation of this group of taxa from its common ancestor occurred after the diversification of other groups. The low cpDNA divergence in this subsection corroborated earlier evidence for its phylogenetic cohesiveness and existence as a monophyletic group.

Key words

Gymnosperms Pinaceae Pinus cpDNA variation molecular systematics evolution Flora of Eurasia 


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  1. Archie, J. W., 1989: Homoplasy excess ratios: new indices for measuring levels of homoplasy in phylogenetic systematics and a critique of the consistency index. — Syst. Zool.38: 253–269.Google Scholar
  2. Bremer, B., 1991: Restriction data from chloroplast DNA for phylogenetic reconstruction: is there only one accurate way of scoring? — Pl. Syst. Evol.175: 39–54.Google Scholar
  3. Burgh, J. van der, 1984: Phylogeny and biogeography of the genusPinus. — InFarjon, A., (Ed.): Pines—drawings and descriptions of the genusPinus, pp. 199–201. — Leiden: Brill, Backhuys.Google Scholar
  4. Critchfield, W. B., Little, Jr, E. L., 1966: Geographic distribution of the pines of the world. — USDA/Forest Service Misc. Publ.991: 1–29.Google Scholar
  5. DeBry, R. W., Slade, N. A., 1985: Cladistic analysis of restriction endonuclease cleavage maps within a maximum-likelihood framework. — Syst. Zool.34: 21–34.Google Scholar
  6. Duffield, J. W., 1952: Relationships and species hybridization in the genusPinus. — Z. Forstgenetik1: 93–97.Google Scholar
  7. El-Kassaby, Y. A., Sigurgeirsson, A., Szmidt, A. E., 1988: The use of restriction analysis of chloroplast DNA in classifying hybrid spruce seedlots. — InHällgren, J.-E., (Ed.): Frans Kempe Symposium: molecular genetics of forest trees, pp. 67–88. — Umeå: Swedish University of Agricultural Sciences.Google Scholar
  8. Farjon, A., 1984: Pines: drawings and descriptions of the genusPinus. — Leiden: Brill, Backhuys.Google Scholar
  9. Felsenstein, J., 1985: Confidence limits on phylogenies: an approach using the bootstrap. — Evolution39: 783–791.Google Scholar
  10. Frankis, M. P., 1988: Generic interrelationships inPinaceae. — Notes Roy. Bot. Gard. Edinburgh45: 527–548.Google Scholar
  11. Govindaraju, D., Lewis, P., Cullis, C., 1992: Phylogenetic analysis of pines using ribosomal DNA restriction fragment length polymorphisms. — Pl. Syst. Evol.179: 141–153.Google Scholar
  12. Karalamangala, R. R., Nickrent, D. L., 1989: An electrophoretic study of representatives of subgenusDiploxylon ofPinus. — Canad. J. Bot.67: 1750–1759.Google Scholar
  13. Klaus, W., 1989: Mediterranean pines and their history. — Pl. Syst. Evol.162: 133–163.Google Scholar
  14. Kluge, A. G., Farris, J. S., 1969: Quantitative phyletics and the evolution of Anurans. — Syst. Zool.18: 1–32.Google Scholar
  15. Kwei, Y. L., Lee, C. L., 1963: Anatomical studies of the leaf structure of Chinese pines. — Acta Bot. Sinica11: 44–58 (in Chinese).Google Scholar
  16. Lidholm, J., Gustafsson, P., 1991: The chloroplast genome of the gymnospermPinus contorta: a physical map and a complete collection of overlapping clones. — Curr. Genet.20: 161–166.PubMedGoogle Scholar
  17. Little, J. E. L., Critchfield, W. B., 1969: Subdivisions of the genusPinus (pines). — USDA Forest Service, Washington D.C., Miscell. Publication No. 1144.Google Scholar
  18. Malusa, J., 1992: Phylogeny and biogeography of thePinyon pines (Pinus subsect.Cembroides). — Syst. Bot.17: 42–66.Google Scholar
  19. McElroy, D., Moran, P., Bermingham, E., Kornfeld, I., 1992: REAP—An integrated environment for the manipulation and phylogenetic analysis of restriction data. — J. Heredity83: 157–158.Google Scholar
  20. Millar, C. I., Strauss, S. H., Conkle, M. T., Westfall, R. D., 1988: Allozyme differentiation and biosystematics of the Californian closed-cone pines (Pinus subsect.Oocarpae). — Syst. Bot.13: 351–370.Google Scholar
  21. Miller, J. C., 1991: Restsite: a phylogenetic program that sorts raw restriction data. — J. Heredity82: 262–263.Google Scholar
  22. Miller, Jr, C. N., 1976: Early evolution in thePinaceae. — Rev. Paleobot. Palynol.21: 101–117.Google Scholar
  23. Mirov, N. T., 1967: The genusPinus. — New York: Ronald Press.Google Scholar
  24. Nei, M., 1987: Molecular evolutionary genetics. — New York: Columbia University Press.Google Scholar
  25. —, 1990: A simple method for estimating average number of nucleotide substitutions within and between populations from restriction data. — Genetics125: 873–879.PubMedGoogle Scholar
  26. Penny, D., Hendy, M. D., Steel, M. A., 1992: Progress with methods for constructing evolutionary trees. — TREE7: 73–79.Google Scholar
  27. Price, R. A., Olsen-Stojkovich, J., Lowenstein, J. M., 1987: Relationships among the genera ofPinaceae: an immunological comparison. — Syst. Bot.12: 91–97.Google Scholar
  28. Rieseberg, L. H., Brunsfeld, S. J., 1992: Molecular evidence and plant introgression. — InSoltis, P. L., Soltis, D. E., Doyle, J. J., (Eds): Molecular systematics of plants, pp. 151–176. — New York, London: Chapman and Hall.Google Scholar
  29. Saitou, N., Nei, M., 1987: The neighbor-joining method: a new method for reconstructing phylogenetic trees. — Mol. Biol. Evol.4: 406–425.PubMedGoogle Scholar
  30. Sandbrink, J. M., Van Brederode, J., 1991: Different approaches to analysis of restriction fragment patterns of chloroplast DNA and phylogenetic relationships inSilene sectionSiphonomorpha (Caryophyllaceae). — Biochem. Syst. Ecol.19: 559–567.Google Scholar
  31. Saylor, L. C., 1972: Karyotype analysis of the genusPinus—subgenusPinus. — Silvae Genet.21: 155–163.Google Scholar
  32. —, 1983: Karyotype analysis of the genusPinus—subgenusStrobus. — Silvae Genet.32: 119–124.Google Scholar
  33. Schaffer, H. E., Sederoff, R., 1981: Improved estimation of DNA fragment lengths from agarose gels. — Analyt. Biochem.115: 113–122.PubMedGoogle Scholar
  34. Shaw, G. R., 1914: The genusPinus. — Publ. Arnold Arbor.5: 1–96.Google Scholar
  35. Shurkhal, A., Podogas, A., Zhivotovki, L., 1992: Allozyme differentiation in the genusPinus. — Silvae Genet.21: 105–109.Google Scholar
  36. Sigurgeirsson, A., 1992: Insights into the evolution ofPicea inferred from chloroplast DNA. — PhD Thesis, Swedish University of Agricultural Sciences, Faculty of Forestry, Department of Forest Genetics and Plant Physiology, Umeå, ISBN 91-576-4617-1.Google Scholar
  37. Strauss, S. H., Doerksen, A. H., 1990: Restriction fragment analysis of pine phylogeny. — Evolution44: 1081–1096.Google Scholar
  38. Sytsma, K. J., Gottlieb, L. D., 1986: Chloroplast DNA evolution and phylogenetic relationships inClarkia sect.Peripetasma (Onagraceae). — Evolution40: 1248–1261.Google Scholar
  39. Szmidt, A. E., 1991: Phylogenetic and applied studies on chloroplast genome in forest conifers. — InFineschi, S., Malvolti, M. E., Cannata, F., Hattemer, H. H., (Eds): Biochemical markers in the population genetics of forest trees, pp. 185–196. — The Hague: Academic Publishing.Google Scholar
  40. —, 1992a: DNA markers in forest genetics. — InMüller-Starck, G., Ziehe, M., (Eds): Genetic variation in forest tree populations in Europe, pp. 79–94. — Frankfurt am Main: Sauerländer.Google Scholar
  41. —, 1993: Molecular systematics and patterns of geographic differentiation in geographic varieties ofPinus sylvestris (L.) andP. densiflora (Sieb. etZucc). — Theor. Appl. Genet.86: 159–165.Google Scholar
  42. —, 1988: Classifying seedlots ofPicea sitchensis andP. glauca in zones of introgression using restriction analysis of chloroplast DNA. — Theor. Appl. Genet.76: 841–845.Google Scholar
  43. —, 1986: DNA extraction and preliminary characterization of chloroplast DNA fromPinus sylvestris andPinus contorta. — InLindgren, D., (Ed.): Frans Kempe Symposium: provenances and forest tree breeding for high latitudes, pp. 269–280. — Umeå: Swedish University of Agricultural Sciences.Google Scholar
  44. —, 1988: Genetic relationships amongPinus species based on chloroplast DNA polymorphism. — InHällgren, J.-E., (Ed.): Frans Kempe Symposium: molecular genetics of forest trees, pp. 33–47. — Umeå: Swedish University of Agricultural Sciences.Google Scholar
  45. Wagner, D. B., Furnier, G. R., Saghai-Maroof, M. A., Williams, S. M., Dancik, B. P., Allard, R. W., 1987: Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids. — Proc. Natl. Acad. Sci. USA84: 2097–2100.PubMedGoogle Scholar
  46. Wang, X.-R., 1992: Genetic diversity and evolution of EurasianPinus species. — PhD thesis, Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology, Umeå, ISBN 91-576-4641-4.Google Scholar
  47. —, 1990: Evolutionary analysis ofPinus densata (Masters), a putative Tertiary hybrid. 2. A study using species-specific chloroplast DNA markers. — Theor. Appl. Genet.80: 641–647.Google Scholar
  48. —, 1991a: Allozyme differentiation among populations ofPinus sylvestris L. from Sweden and China. — Hereditas114: 219–226.Google Scholar
  49. —, 1991b: A chloroplast DNA story about Asiatic pines. — InFineschi, S., Malvolti, M. E., Cannata, F., Hattemer, H. H., (Eds): Biochemical markers in the population genetics of forest trees, pp. 209–216. — The Hague: Academic Publishing.Google Scholar
  50. Wheeler, N. C., Guries, R. P., O'Malley, D. M., 1983: Biosystematics of the genusPinus, subsectionContortae. — Biochem. Syst. Ecol.11: 333–340.Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Xiao-Ru Wang
    • 1
  • Alfred E. Szmidt
    • 1
  1. 1.Department of Forest Genetics and Plant PhysiologySwedish University of Agricultural SciencesUmeåSweden

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