Advertisement

Plant Systematics and Evolution

, Volume 179, Issue 3–4, pp 221–233 | Cite as

Genotype relationships inMicroseris elegans (Asteraceae, Lactuceae) revealed by DNA amplification from arbitrary primers (RAPDs)

  • A. W. van Heusden
  • K. Bachmann
Article

Abstract

The genetic relationships among 10 inbred lines representing 10 populations of the autogamous annualMicroseris elegans from throughout California has been determined using random amplified polymorphic DNAs (RAPDs). Seventeen arbitrary 10 base pair primers produced 134 amplification products; 81 of these were shared by two or more strains. The 3 genotypes from Northern California are closely related as are 3 genotypes from Middle Californian populations which are not nearest neighbors. DNA fingerprinting with the oligonucleotide (GATA)4 gave compatible results, but the comparison was limited to samples run on one gel. Isoenzyme patterns are compatible with the DNA results, but limited by the very low number of informative polymorphisms. The clustered relationship among genotypes within a species and their geographic distribution suggests very restricted genetic recombination and an origin of new populations from randomly dispersed achenes within the range of the species.

Key words

Angiosperms Asteraceae Microseris Phylogeny RAPDs DNA fingerprinting isoenzymes dispersal distribution 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ali, S., Müller, C. R., Epplen, J. T., 1986: DNA fingerprinting by oligonucleotide probes specific for simple repeats. — Hum. Genet.74: 239–243.Google Scholar
  2. Bachmann, K., Chambers, K. L., 1990a: Heritable variation for heterocarpy inMicroseris bigelovii (Asteraceae—Lactuceae). — Beitr. Biol. Pflanzen65: 123–146.Google Scholar
  3. —, —, 1990b: Genetic variation for the timing and site of trichomes on the leaves ofMicroseris bigelovii (Asteraceae: Lactuceae). — Biol. Zentralbl.109: 151–158.Google Scholar
  4. —, —,Grau, J., Price, H. J., 1985a: Genetic variation inMicroseris pygmaea (Asteraceae—Lactuceae). — Beitr. Biol. Pflanzen60: 51–88.Google Scholar
  5. —, —,Price, H. J., 1979: Genome size and phenotypic variation inMicroseris (Asteraceae, Cichorieae). — Pl. Syst. Evol. Suppl.2: 41–66.Google Scholar
  6. —, —, —, 1984: Differential geographic distribution of spatulate and pointed leaf shapes inMicroseris bigelovii (Asteraceae—Lactuceae). — Beitr. Biol. Pflanzen59: 5–14.Google Scholar
  7. —, —, —, 1982: Four additive genes determining pappus part numbers inMicroseris annual hybrid C34 (Asteraceae/Lactuceae). — Pl. Syst. Evol.141: 123–141.Google Scholar
  8. —,Heusen, A. W. van, Chambers, K. L., Price, H. J., 1985b: Duplications of additively acting genes in the evolution of a plant (Microseris pygmaea). — Experientia41: 1348–1350.Google Scholar
  9. —, —, —, —, 1987: Genetic variation for the onset of flowering inMicroseris bigelovii (Asteraceae, Lactuceae). — Beitr. Biol. Pflanzen62: 23–41.Google Scholar
  10. Chambers, K. L., 1955: A biosystematic study of the annual spocies ofMicroseris. — Contrib. Dudley Herbarium4: 207–312.Google Scholar
  11. Dellaporta, S. L., Wood, J., Hicks, J. B., 1983: A plant DNA minipreparation: version II. — Plant Mol. Biol. Reporter1: 19–21.Google Scholar
  12. Epplen, J. T., 1988: On simple repeated GAt/cA sequences in animal genomes: a critical reappraisal. — J. Heredity79: 409–417.Google Scholar
  13. Felsenstein, J., 1985: Confidence limits on phylogenies: an approach using the bootstrap. — Evolution39: 783–791.Google Scholar
  14. Gebhardt, C., Blomendahl, C., Schachtschabel, U., Debener, T., Salamini, F., Ritter, E., 1989: Identification of 2n breeding lines and 4n varieties of potato (Solanum tuberosum, ssp.tuberosum) with RFLP fingerprints. — Theor. Appl. Genet.78: 16–22.Google Scholar
  15. Gilbert, D. A., Lehman, N., O'Brien, S. J., Wayne, R. K., 1990: Genetic fingerprinting reflects population differentiation in the California Channel Island fox. — Nature344: 764–767.Google Scholar
  16. Heusden, A. W. van, 1990: Genetic analysis of natural variation inMicroseris pygmaea (Asteraceae) in crosses withM. bigelovii C93b. — Thesis, Amsterdam.Google Scholar
  17. —,Rouppe van der Voort, J., Bachmann, K., 1991: Oligo-(GATA) fingerprints identify clones in asexual dandelions (Taraxacum, Asteraceae). — Fingerprint News3(2: 13–15.Google Scholar
  18. Houten, W. H. J. van, Heusden, A. W. van, Rouppe van der Voort, J., Raijmann, L., Bachmann, K., 1991: Hypervariable DNA fingerprint loci inMicroseris pygmaea (Asteraceae, Lactuceae). — Bot. Acta104: 252–256.Google Scholar
  19. Jeffreys, A. J., Wilson, V., Thein, S. L., 1985: Individual specific fingerprints of human DNA. — Nature316: 76–79.Google Scholar
  20. Maniatis, T., Fritsch, E. F., Sandbrook, J., 1982: Molecular cloning: a laboratory manual. — New York: Cold Spring Harbor Laboratory.Google Scholar
  21. Nybom, H., Rogstad, S. H., 1990: DNA “fingerprints” detect genetic variation inAcer negundo (Aceraceae). — Pl. Syst. Evol.173: 49–56.Google Scholar
  22. —, —,Schaal, B. A., 1990: Genetic variation detected by use of the M13 “DNA fingerprint” probe inMalus, Prunus, andRubus (Rosaceae). — Theor. Appl. Genet.79: 153–156.Google Scholar
  23. —,Schaal, B. A., Rogstad, S. H., 1989: DNA “fingerprints” can distinguish cultivars of blackberries and raspberries. — Acta Hortic.262: 310–315.Google Scholar
  24. Sneath, P. H. A., Sokal, R. R., 1973: Numerical taxonomy. — San Francisco: Freeman.Google Scholar
  25. Wallace, R. S., Jansen, R. K., 1990: Systematic implications of chloroplast DNA variation in the genusMicroseris (Asteraceae: Lactuceae). — Syst. Bot.15: 606–616.Google Scholar
  26. Weising, K., Kahl, G., 1990: DNA fingerprinting in plants — the potential of a new method. — Biotech-Forum Europe7: 230–235.Google Scholar
  27. Williams, J. K. G., Kubelik, A. R., Litvak, K. J., Rafalski, J. A., Tingey, S. V., 1990: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. — Nucleic Acids Res.18: 6531–6535.Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • A. W. van Heusden
    • 1
  • K. Bachmann
    • 1
  1. 1.Hugo de Vries LaboratoryAmsterdamThe Netherlands

Personalised recommendations