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Comparison of in vivo and in vitro phagocytosis inGalleria mellonella L.

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Abstract

Under both in vivo and in vitro conditions, haemocytes ofGalleria mellonella engulf bacteria, yeast and abiotic particles, whereby a large proportion of haemocytes are engaged in this reaction. Following the inoculation of particles, some are cleared from the circulation but not phagocytosed. Simultaneous injection of these particles and eitherN-acetyl-d-glucosamine ord-mannose causes an increase in the proportion of phagocytosing haemocytes, which is assumed to be mediated by inhibition of their agglutination.

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Abbreviations

BTS:

Bacillus thuringiensis subtoxicus

HRBC :

human red blood cells

SEM :

scanning electron microscopy

SRBC :

sheep red blood cells

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Ehlers, D., Zosel, B., Mohrig, W. et al. Comparison of in vivo and in vitro phagocytosis inGalleria mellonella L.. Parasitol Res 78, 354–359 (1992). https://doi.org/10.1007/BF00937096

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