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Adherence betweenEntamoeba histolytica trophozoites and undifferentiated or DMSO-induced HL-60 cells

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Abstract

Differentiation of promyelocytic HL-60 cells along the pathway toward granulocytes using dimethylsulfoxide (DMSO) led to increased expression of the adhesion molecules CD11b (Mac-1), CD11c (p150,95), and CD35 (CR1). Undifferentiated and differentiated HL-60 cells adhered similarly toEntamoeba histolytica trophozoites, and monoclonal antibodies against adhesion molecules CD11b and CD11c did not inhibit adherence. We therefore suggest that CD11b and CD11c are not involved in the adherence between polymorphonuclear granulocytes andE. histolytica trophozoites.

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References

  • Arroyo R, Orozco E (1987) Localization and identification of anEntamoeba histolytica adhesin. Mol Biochem Parasitol 23:151–158

    PubMed  Google Scholar 

  • Brattig NW, Medina-de-la-Garza CE, Tischendorf FW (1987) Comparative study of eosinophil purification on Nycodenz, Metrizamide and Percoll density gradients. Eur J Haematol 39:148–153

    PubMed  Google Scholar 

  • Bullock WE, Wright SD (1987) The role of adherence-promoting receptors, CR3, LFA-1, and p150/95 in binding ofHistoplasma capsulatum by human macrophages. J Exp Med 165:195–210

    PubMed  Google Scholar 

  • Burchard GD, Bilke R (1992) Adherence of pathogenic and non-pathogenicEntamoeba histolytica to neutrophils. Parasitol Res 78:146–153

    PubMed  Google Scholar 

  • Chadee K, Ndarathi C, Keller K (1990) Binding of proteolytically degraded human colonic mucin glycoproteins to the Gal/Gal-NAc adherence lectin ofEntamoeba histolytica. Gut 31:890–895

    PubMed  Google Scholar 

  • Clark CG, Diamond LS (1991) Ribosomal RNA genes of “pathogenic” and “nonpathogenic”Entamoeba histolytica are distinct. Mol Biochem Parasitol 49:297–302

    PubMed  Google Scholar 

  • Collins SJ (1987) The HL-60 promyelocytic leukaemia cell line: proliferation, differentiation, and cellular oncogene expression. Blood 70:1233–1244

    PubMed  Google Scholar 

  • Diamond LS, Harlow DR, Cunnick CC (1978) A new medium for the axenic cultivation ofEntamoeba histolytica and otherEntamoeba. Trans R Soc Trop Med Hyg 72:431–432

    PubMed  Google Scholar 

  • Dudley D, Baker DM, Hickey MJ, Hickstein DD (1989) Expression of surface antigen and mRNA for the CD11c (alpha X, p150) subunit of the human leukocyte adherence receptor family in hematopoietic cells. Biochem Biophys Res Commun 160:346–353

    PubMed  Google Scholar 

  • Guerrant RL, Brush J, Ravdin JI, Sullivan JA, Mandell GL (1981) Interaction betweenE. histolytica and human polymorphonuclear neutrophils. J Infect Dis 143:83–93

    PubMed  Google Scholar 

  • Hickstein DD, Back AL, Collins SJ (1989) Regulation of expression of the CD11b and CD18 subunits of the neutrophil adherence receptor during human myeloid differentiation. J Biol Chem 264:21812–21817

    PubMed  Google Scholar 

  • Levy R, Rotrosen D, Nagauker O, Leto TL, Malech HL (1990) Induction of the respiratory burst in HL-60 cells. Correlation of function and protein expression. J Immunol 145:2595–2601

    PubMed  Google Scholar 

  • Lotan R, Lotan D, Carralero DM (1989) Modulation of galacioside-binding lectins in tumor cells by differentiation-inducing agents. Cancer Lett 48:115–122

    PubMed  Google Scholar 

  • Mann BJ, Vedvick T, Torian B, Petri WA (1991) Cloning of the 170 kDa subunit of the galactose-specific adherence lectin ofEntamoeba histolytica. Proc Natl Acad Sci USA 88:3248–3252

    PubMed  Google Scholar 

  • Martínez-Palomo A, Tsutsumi V, Anaya-Velazques F, Gonzalez-Robles A (1989) Ultrastructure of experimental intestinal invasive amebiasis. Am J Trop Med Hyg 41:273–279

    PubMed  Google Scholar 

  • Meza I, Cázares F, Rosales-Encina JL, Talamas-Rohana P, Rojkind M (1987) Use of antibodies to characterize a 220-kilodalton surface protein fromEntamoeba histolytica. J Infect Dis 156:798–805

    PubMed  Google Scholar 

  • Newburger PE, Chovaniec ME, Greenberger JS, Cohen HJ (1979) Functional changes in human leucemic cell line HL-60. J Cell Biol 82:315–322

    PubMed  Google Scholar 

  • Petri WA, Chapman MD, Snodgrass T, Mann BJ, Broman J, Ravdin JI (1989) Subunit structure of the galactose andN-acetyl-d-galactosamine-inhibitable adherence lectin ofEntamoeba histolytica. J Biochem Chem 264:3007–3012

    Google Scholar 

  • Prathap K, Gilman R (1970) The histopathology of acute intestinal amebiasis. A rectal biopsy study. Am J Pathol 60:229–246

    PubMed  Google Scholar 

  • Ravdin JI, Guerrant RL (1981) The role of adherence in cytopathogenic mechanisms ofE. histolytica. Study with mammalian tissue culture cells and human red blood cells. J Clin Invest 68:1305–1313

    PubMed  Google Scholar 

  • Ravdin JI, John JE, Johnston KI, Innes DJ, Guerrant RL (1985) Adherence ofEntamoeba histolytica trophozoites to rat and human colonic mucosa. Infect Immun 48:292–297

    PubMed  Google Scholar 

  • Rosales-Encina JL, Meza I, López-de-León A, Talamás-Rohana P, Rojkind M (1987) Isolation of a 220-kilodalton protein with lectin properties from a virulent strain ofEntamoeba histolytica. J Infect Dis 156:790–797

    PubMed  Google Scholar 

  • Rosmarin AG, Wei SC, Rosner GL, Griffin JD, Arnaout MA, Tenen DG (1989) Differential expression of CD11b/CD18 (Mo1) and myeloperoxidase genes during myelod differentiation. Blood 73:131–136

    PubMed  Google Scholar 

  • Saffer LD, Petri WA (1991)Entamoeba histolytica: recognition of α- and β-galactose by the 260-kDa adherence lectin. Exp Parasitol 72:106–108

    PubMed  Google Scholar 

  • Salata RA, Ravdin JI (1988) The interaction of polymorphonuclear neutrophils andEntamoeba histolytica. In: Ravdin JI (ed) Amebiasis. Churchill Livingstone, Edinburgh New York, pp 464–470

    Google Scholar 

  • Schnur RA, Newman SL (1990) The respiratory burst response toHistoplasma capsulatum by human neutrophils. Evidence for intracellular trapping of superoxide anion. J Immunol 144:4765–4772

    PubMed  Google Scholar 

  • Tannich E, Burchard GD (1991) Differentiation of pathogenic from nonpathogenicEntamoeba histolytica by restriction fragment analysis of a single gene amplified in vitro. J Clin Microbiol 29:250–255

    PubMed  Google Scholar 

  • Tannich E, Ebert F, Horstmann RD (1991) Primary structure of the 170-kDa surface lectin of pathogenicEntamoeba histolytica. Proc Natl Acad Sci USA 88:1849–1853

    PubMed  Google Scholar 

  • Tsutsumi V, Martínez-Palomo A (1988) Inflammatory reaction in experimental hepatic amebiasis. An ultrastructural study. Am J Pathol 130:112–119

    PubMed  Google Scholar 

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Burchard, G.D., Möslein, C. & Brattig, N.W. Adherence betweenEntamoeba histolytica trophozoites and undifferentiated or DMSO-induced HL-60 cells. Parasitol Res 78, 336–340 (1992). https://doi.org/10.1007/BF00937093

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