Inhibitory effect ofTrypanosoma brucei brucei onGlossina morsitans midgut trypsin in vitro
The ability ofTrypanosoma brucei brucei to inhibit trypsin or trypsin-like enzymes in crude midgut homogenates ofGlossina morsitans morsitans was studied in vitro. The isolated parasites caused a concentration-dependent decrease in midgut trypsin activity. Furthermore, trypanosomes lysed by repeated freeze-thawing had a similar effect on trypsin activity. In both cases, the inhibition by either intact or lysed parasites was partial as revealed by Dixon plots. Similarly, trypanosome membrane proteins stoichiometrically inhibited trypsin activity, suggesting that the enzyme interacts specifically with a moiety on the parasite surface. The Km and Ki values obtained in this case were 35 μm and 0.18 mg/ml, respectively. These results suggest that one of the ways in which trypanosomes overcome the hostile tsetse-fly midgut barrier involves the inhibition of enzyme activity.
KeywordsEnzyme Activity Trypsin Membrane Protein Trypsin Activity Inhibit Trypsin Activity
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