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DNA sequence analyses of the ribosomal spacer regions in theTriticeae

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Abstract

Two regions of the ribosomal DNA (rDNA) were sequenced from a range of species from the tribeTriticeae. One region, the central spacer, was found to be more divergent in sequence than the other, the 18 S-spacer junction. Both regions contained sequences 20–30 bp long which were more highly conserved than the remainder of the region and their possible significance in rDNA expression is discussed. Phenetic relationships based on the sequence data were generally consistent with the relationships based on other criteria. Species possessing the S, E, J1J2, D, and B genomes clustered together, with the H genome species being the most distinct of those examined. The R, P, and V genome species occupy an intermediate position in the overall pattern of relationships. Some relationships differed in detail from those established by other parameters, for example the position of the N genome species, and explanations for discrepancies of this type are discussed.

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References

  • Appels, R., Dvorak, J., 1982: The wheat ribosomal DNA spacer region: its structure and variation in populations and among species. — Theor. Appl. Genet.63: 337–348.

    Google Scholar 

  • —,McIntyre, C. L., Clarke, B. C., May, C. E., 1986a: Alien chromatin in wheat: ribosomal DNA spacer probes for detecting specific nucleolar organizer region loci introduced into wheat. — Canad. J. Genet. Cytol.28: 665–672.

    Google Scholar 

  • —,Moran, L. B., Gustafson, J. P., 1986b: The structure of DNA from the rye (Secale cereale)Nor R 1 locus and its behaviour in wheat backgrounds. — Canad. J. Genet. Cytol.28: 673–685.

    Google Scholar 

  • Arnheim, N., 1983: Concerted evolution in multigene families. — InKoehn, R., Nei, M., (Eds.): Evolution of genes and proteins, pp. 38–61. — Sunderland, Mass.: Sinauer Associates Inc.

    Google Scholar 

  • Bach, R., Allet, B., Crippa, M., 1981: Sequence organization of the spacer in the ribosomal genes ofXenopus clivii andXenopus borealis. — Nucleic Acids Res.9: 5311–5320.

    Google Scholar 

  • Brettell, R. I. S., Pallotta, M. A., Gustafson, J. P., Appels, R., 1986: Variation at theNOR loci in triticale derived from tissue culture. — Theor Appl. Genet.71: 637–643.

    Google Scholar 

  • Brown, D. D., Wensink, P. C., Jordan, E., 1972: A comparison of the ribosomal DNA's ofXenopus laevis andXenopus mulleri: the evolution of tandem genes. — J. Mol. Biol.63: 57–73.

    Google Scholar 

  • Feldman, M., Sears, E. R., 1981: The wild gene resources of wheat. — Scientific American244: 102–112.

    Google Scholar 

  • Flavell, R. B., O'Dell, M., Sharp, P., Nevo, E., Beiles, A., 1986: Variation in the intergenic spacer of ribosomal DNA of wild wheat,Triticum dicoccoides, in Israel. — Mol. Biol. Evol.3: 547–558.

    Google Scholar 

  • Gerlach, W. L., Dyer, T. A., 1980: Sequence organization of the repeating units in the nucleus of wheat which contain 5 S rRNA genes. — Nucleic Acids Res.8: 4851–4865.

    Google Scholar 

  • Gill, B. S., Appels, R., 1988: Relationships betweenNor-loci from differentTriticeae species. — Pl. Syst. Evol.160: 77–89.

    Google Scholar 

  • —,Morris, K. L. D., Appels, R., 1988: Assignment of the genomic affinities of chromosomes from polyploidElymus species added to wheat. — Genome30: 70–82.

    Google Scholar 

  • Grummt, I., Roth, E., Paule, M. R., 1982: Ribosomal RNA transcription in vitro is species-specific. — Nature296: 173–174.

    Google Scholar 

  • Lassner, M., Dvorak, J., 1986: Preferential homogenization between adjacent and alternate subrepeats in wheat rDNA. — Nucleic Acids Res.14: 5499–5512.

    Google Scholar 

  • —,Anderson, O., Dvorak, J., 1987: Inferences on intergenomic homogenization based on the molecular structure of a cloned ribosomal RNA gene from the D-genome of bread wheat. — Genome29: 770–781.

    Google Scholar 

  • McIntyre, C. L., 1988: Variation at isozyme loci inTriticeae. — Pl. Syst. Evol.160: 123–142.

    Google Scholar 

  • McMullen, M. D., Hunter, B., Phillips, R. L., Rubenstein, I., 1986: The structure of the maize ribosomal RNA spacer region. — Nucleic Acids Res.14: 4953–4968.

    Google Scholar 

  • May, C. E., Appels, R., 1987: Variability and genetics of spacer DNA sequences between the ribosomal-RNA genes of hexaploid wheat (Triticum aestivum). — Theor. Appl. Gen.74: 617–624.

    Google Scholar 

  • Messing, J., Crea, R., Seeburg, P. H., 1981: A system for shotgun DNA sequencing. — Nucleic Acids Res.9: 309–321.

    Google Scholar 

  • Mishima, Y., Financsek, I., Kominami, R., Muramatsu, M., 1982: Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor. — Nucleic Acids Res.10: 6659–6670.

    Google Scholar 

  • Reeder, R. H., Roan, J. G., Dunaway, M., 1983: Spacer regulation ofXenopus ribosomal gene transcription: competition in oocytes. — Cell35: 440–456.

    Google Scholar 

  • Sanger, F., Nicklen, S., Coulson, A. R., 1977: DNA sequencing with chain-terminating inhibitors. — Proc. Natl. Acad. Sci., U.S.A.74: 5463–5467.

    Google Scholar 

  • Scoles, G. J., Gill, B. S., Xin, Z.-Y., Clarke, B. C., McIntyre, C. L., Chapman, C., Appels, R., 1988: Frequent duplication and deletion events in the 5 S RNA genes and the associated spacer regions of theTriticeae. — Pl. Syst. Evol.160: 105–122.

    Google Scholar 

  • Simeone, A., la Volpe, A., Boncinelli, E., 1985: Nucleotide sequence of a complete ribosomal spacer ofD. melanogaster. — Nucleic Acids Res.13: 1089–1101.

    Google Scholar 

  • Smith, D. K., 1987: Concept and use of software for pair-wise and multiple genetic sequence alignment. — Technical Report No.11: Canberrra, CAE, ACT, Australia.

  • Snape, J. W., Flavell, R. B., O'Dell, M., Hughes, W. G., Payne, P. I., 1984: Intrachromosomal mapping of the nucleolar organizer region relative to three marker loci on chromosome 1 B of wheat (Triticum aestivum). — Theor. Appl. Genet.69: 263–270.

    Google Scholar 

  • Toloczyki, C., Feix, G., 1986: Occurrence of 9 homologous repeat units in the external spacer region of a nuclear maize rDNA gene unit. — Nucleic Acids Res.14: 4969–4986.

    Google Scholar 

  • Xin, Z.-Y., Appels, R, 1988: Occurrence of rye (Secale cereale) 350-family DNA sequences inAgropyron and otherTriticeae. — Pl. Syst. Evol.160: 65–76.

    Google Scholar 

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McIntyre, C.L., Clarke, B.C. & Appels, R. DNA sequence analyses of the ribosomal spacer regions in theTriticeae . Pl Syst Evol 160, 91–104 (1988). https://doi.org/10.1007/BF00936712

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