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Amplification and dispersion of repeated DNA sequences in theTriticeae

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Abstract

Four representatives of a family of dispersed repetitive sequences which were prominent and dispersed in the E genome ofThinopyrum elongatum but poorly represented in wheat, were studied in detail. The 1.4kb sequences were present both as part of tandem and more complex arrays and appeared to have resulted from repeated amplification of the sequence and their dispersion throughout the genome. Subcloning of sections of the 1.4 kb sequences resulted in probes which improved the resolution of the E genome from the genomes in wheat and enabled identification of single E genome chromosomes introduced into wheat. The generality of these types of sequences in the tribeTriticeae was confirmed by isolating analogous sequences from the R (rye,Secale cereale), V (Dasypyrum villosum), and N (Psathyrostachys juncea) genomes. — The cloned repetitive sequences from the R, V, and N genomes each showed characteristic fluctuations in amount within the grasses examined in addition to being virtually absent from wheat. It is thus possible that these sequences may provide useful taxonomic indicators for establishing relationships within theTriticeae, as well as valuable probes for tracing alien chromatin introduced into wheat.

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  1. Division of Plant Industry, CSIRO, P.O. Box 1600, 2601, Canberra, ACT, Australia

    C. L. McIntyre, B. C. Clarke & R. Appels

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  1. C. L. McIntyre
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McIntyre, C.L., Clarke, B.C. & Appels, R. Amplification and dispersion of repeated DNA sequences in theTriticeae . Pl Syst Evol 160, 39–59 (1988). https://doi.org/10.1007/BF00936708

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