Phosphorylation ofToxoplasma gondii major surface antigens
The phosphorylation of the major surface proteins ofToxoplasma gondii tachyzoites was investigated. Metabolic labeling of intracellular tachyzoites with [32P]-orthophosphate followed by immunoprecipitation with specific monoclonal antibodies showed that all five major surface proteins were labeled as expected for GPI-anchored proteins. More detailed analysis of the phosphorylated sites revealed that surface proteins P30 and P22 contained phosphoserine residues in addition to the phosphorylated molecules that are presumably localized in the GPI-membrane anchors
KeywordsOrthophosphate Monoclonal Antibody Detailed Analysis Surface Protein Surface Antigen
bovine serum albumin
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