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Parasitology Research

, Volume 78, Issue 7, pp 541–544 | Cite as

Phosphorylation ofToxoplasma gondii major surface antigens

  • Stanislas Tomavo
  • Arlette Martinage
  • Jean François Dubremetz
Original Investigations

Abstract

The phosphorylation of the major surface proteins ofToxoplasma gondii tachyzoites was investigated. Metabolic labeling of intracellular tachyzoites with [32P]-orthophosphate followed by immunoprecipitation with specific monoclonal antibodies showed that all five major surface proteins were labeled as expected for GPI-anchored proteins. More detailed analysis of the phosphorylated sites revealed that surface proteins P30 and P22 contained phosphoserine residues in addition to the phosphorylated molecules that are presumably localized in the GPI-membrane anchors

Keywords

Orthophosphate Monoclonal Antibody Detailed Analysis Surface Protein Surface Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BSA

bovine serum albumin

GPI

glycosyl-phosphatidylinositol

mAb

monoclonal antibody

NP-40

Nonidet P-40

PBS

phosphate-buffered saline

PMSF

phenylmethylsulfonylfluoride

TCA

trichloroacetic acid

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Stanislas Tomavo
    • 1
  • Arlette Martinage
    • 2
  • Jean François Dubremetz
    • 1
  1. 1.Unité 42 INSERMVilleneuve D'AscqFrance
  2. 2.Unité 409 CNRSInstitut de recherche sur le CancerLilleFrance

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