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Molecular and Cellular Biochemistry

, Volume 125, Issue 2, pp 145–151 | Cite as

Modulation of allo-immune responsesin vivo andin vitro by sperm specific lactate dehydrogenase-C4

  • G. S. Gupta
  • R. G. Kinsky
Article

Abstract

The role of sperm specific lactate dehydrogenase-C4 (LDH-C4) in allo-immune responses using mixed lymphocyte cultures (MLC) and cytotoxic T cell (CTL) generationin vitro and local graft versus host (LGVH) reaction and allograft enhancementin vivo has been ascertained. LDH was purified from testes (LDH-C4) and kidney (LDH-B4) of C57 Bl/Ks mice. MLC and CTL were performed using C57 Bl/Ks-anti A/J lymphocytes in presence of 10−3-1 μg LDH-B4 or LDH-C4 per culture. The MLC and CTL responses showed biphasic action depending on the dose of LDH-C4. Early MLC culture gave significantly low stimulation index at 10−2–10−1 μg LDH-C4 as compared to non-treated control cultures. However, the MLC response in presence of LDH-C4 was not different from the LDH-B4 treated one which showed a similar biphasic trend. On the other hand,51Cr release from YAC-222 target cells was practically abolished by LDH-C4 at 10−3–1−1 μg, and this was strikingly different from LDH-B4 or non-treated cultures. LGVH reactivity as performed by using C57 Bl/Ks lymphocytes along with LDH-C4 in (C57 Bl/Ks x A/J) F1 hybrids indicated a suppression of stimulation index in primary and secondary (i.e. preimmunized in presence of LDH-C4 or LDH-B4) LGVH. Allograft enhancement of Sa I (A/J) in C57 Bl/Ks mice in presence of LDH-C4, was delayed slightly but significantly during primary or secondary transplantation reaction. The reaction of LDH-C4 in the modulation of allo-immune responses was more specificin vivo thanin vitro since the B4 isozyme did not modify LGVH and Sa1 allograft rejection. Resultsper se suggest that LDH-C4 is immunosuppressive for cell mediated allo-immune responsesin vivo andin vitro.

Key words

LDH-isozymes sperm specific LDH immunesuppression allo-immune responses MLC CTL generation LGVH reaction PLN assay allograft rejection 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • G. S. Gupta
    • 1
    • 2
  • R. G. Kinsky
    • 1
    • 2
  1. 1.Department of BiophysicsPanjab UniversityChandigarhIndia
  2. 2.INSERM U 262Baudelocque HospitalParisFrance

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