Molecular and Cellular Biochemistry

, Volume 125, Issue 2, pp 145–151 | Cite as

Modulation of allo-immune responsesin vivo andin vitro by sperm specific lactate dehydrogenase-C4

  • G. S. Gupta
  • R. G. Kinsky


The role of sperm specific lactate dehydrogenase-C4 (LDH-C4) in allo-immune responses using mixed lymphocyte cultures (MLC) and cytotoxic T cell (CTL) generationin vitro and local graft versus host (LGVH) reaction and allograft enhancementin vivo has been ascertained. LDH was purified from testes (LDH-C4) and kidney (LDH-B4) of C57 Bl/Ks mice. MLC and CTL were performed using C57 Bl/Ks-anti A/J lymphocytes in presence of 10−3-1 μg LDH-B4 or LDH-C4 per culture. The MLC and CTL responses showed biphasic action depending on the dose of LDH-C4. Early MLC culture gave significantly low stimulation index at 10−2–10−1 μg LDH-C4 as compared to non-treated control cultures. However, the MLC response in presence of LDH-C4 was not different from the LDH-B4 treated one which showed a similar biphasic trend. On the other hand,51Cr release from YAC-222 target cells was practically abolished by LDH-C4 at 10−3–1−1 μg, and this was strikingly different from LDH-B4 or non-treated cultures. LGVH reactivity as performed by using C57 Bl/Ks lymphocytes along with LDH-C4 in (C57 Bl/Ks x A/J) F1 hybrids indicated a suppression of stimulation index in primary and secondary (i.e. preimmunized in presence of LDH-C4 or LDH-B4) LGVH. Allograft enhancement of Sa I (A/J) in C57 Bl/Ks mice in presence of LDH-C4, was delayed slightly but significantly during primary or secondary transplantation reaction. The reaction of LDH-C4 in the modulation of allo-immune responses was more specificin vivo thanin vitro since the B4 isozyme did not modify LGVH and Sa1 allograft rejection. Resultsper se suggest that LDH-C4 is immunosuppressive for cell mediated allo-immune responsesin vivo andin vitro.

Key words

LDH-isozymes sperm specific LDH immunesuppression allo-immune responses MLC CTL generation LGVH reaction PLN assay allograft rejection 


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  1. 1.
    Gupta GS, Goldberg E: Isolation, properties, immunological specificity and localization of mouse testicular hyaluronidase. Biochim Biophys Acta 657: 364–373, 1981Google Scholar
  2. 2.
    Goldberg E: Effect of immunization with LDH-X on fertility. Acta Endocrinol (Suppl) 78: 202–222, 1975Google Scholar
  3. 3.
    Tinnberg H, Birke R, Mettler L: Effect of fertility of female mice of the transfer for lymphocytes from females previously immunized with spermatozoa. J Reprod Fertil 58: 469–473, 1980Google Scholar
  4. 4.
    Voisin GA, Toullet F, D'Almeida M: Characterization of spermatozoal auto, iso and alloantigens. Acta Endocrinol (Suppl) 78: 173–201, 1975Google Scholar
  5. 5.
    Fellous M, Erickson RP, Gachelin C, Jacobs F: Time appearance of la antigens during spermatogenesis in mouse. Transplantation 22: 440–444, 1976Google Scholar
  6. 6.
    Bulmer JN, Billington WD, Johnson PM: Immunohistologic identification of trophoblast populations in early human pregnancy with the use of monoclonal antibodies. Am J Obstet Gynecol 48: 19–26, 1984Google Scholar
  7. 7.
    Bulmer JN, Johnson PM, Bulmer D: Leukocyte populations in human deciduda and endometrium. In: TJ Gill, TG Wegmann (eds) Immunoregulation and fetal survival. Oxford University Press, New York, 1987, pp 111–134Google Scholar
  8. 8.
    Hill JA, Haimovici F, Anderson DJ: Products of activated lymphocytes and macrophages inhibit mouse embryo developmentin vitro. J Immunol 138: 2250–2254, 1987Google Scholar
  9. 9.
    Hurtenbach U, Shearer GM: Germ cell induced immune suppression in mice. Effect of inoculation of syngeneic spermatozoa on cell mediated immune responses. J Expt Med 155: 1719–1729, 1982Google Scholar
  10. 10.
    James K, Hargreave TB: Immunosuppression by seminal plasma and its possible clinical significance. Immunology Today 5: 357–363, 1984Google Scholar
  11. 11.
    Alexander NJ, Anderson DJ: Immunology of semen. Fertil Steril 47: 192–205, 1987Google Scholar
  12. 12.
    Goldberg E: Developmental expression of lactate dehydrogenase isozymes during spermatogenesis. Prog Clin Biol Res 344: 49–52, 1990Google Scholar
  13. 13.
    Sakai I, Sharief F, Li SS-L: Molecular cloning and nucleotide sequence of c-DNA for sperm specific lactate dehydrogenase-C from mouse. Biochem J 24: 619–622, 1987Google Scholar
  14. 14.
    Alvarej JG, Stoery BT: Assessment of cell damage caused by spontaneous lipid peroxidation in rabbit sperm. Biol Repord 30: 323–331, 1984Google Scholar
  15. 15.
    Gupta GS, Kinsky RG, Couderc J: Modulation of T cell functions by sperm specific lactate dehydrogenase: Fluorescence analysis of immune competent cells and local graft versus host reaction. Ind J Biochem Biophys 29: 271–276, 1992Google Scholar
  16. 16.
    Lee C-Y, Yuan JH, Goldberg E: Lactate dehydrogenase isozymes form mouse. Meth Enzymol 89: 351–358, 1982Google Scholar
  17. 17.
    Gupta GS, Kinsky RG, Voisin GA: Effect of placental glycoproteins on allo-antibody formation, Host-versus graft reaction and mixed lymphocyte reactivity. Ind J Expt Biol 25: 805–810, 1987Google Scholar
  18. 18.
    Cerottini JC, Nordin AA, Burnner KT:In vitro cytotoxic activity of thymus cells sensitized to alloantigens. Nature 227: 72–73, 1970Google Scholar
  19. 19.
    Gupta GS, Kinsky RG, Duc HT, Voisin GA: Effects of placental extracts on the immune response to histocompatibility antigens: Class deviation of allo-antibody response and allograft enhancement. Am J Reprod Immunol 6: 117–123, 1984Google Scholar
  20. 20.
    Droge W, Roth S, Altman A, Mihm S: Regulation of T cell functions by L-lactate. Cell Immunol 108: 405–416, 1987Google Scholar
  21. 21.
    Berger NA, Berger SJ, Sikorski GW, Catino DM: Amplification of pyridine nucleotide pools in mitogen-stimulated human lymphocytes. Expt Cell Res 137: 79–88, 1982Google Scholar
  22. 22.
    Gupta GS, Kinsky RG: Immunoregulatory properties of lactate dehydrogenase-C4in vivo and its effect on embryo resorption. Human Reprod 3 (Suppl): 120, 1988Google Scholar
  23. 23.
    Gupta GS, Kapur S, Kinsky RG: Inhibition kinetics of lactate dehydrogenase isozymes by gossypol acetic acid. Biochem Inter 17: 25–34, 1988Google Scholar
  24. 24.
    Gupta GS, Kinsky RG: Identification of murine placental glycoproteins in relation toin vivo andin vitro immunomodulatory properties against H-2 antigen. Nephrologie 9: 167–175, 1988Google Scholar
  25. 25.
    Drake BL, Rodger JC: Thein vitro immunomodulatory properties of cultured murine trophoblasts are not unique to histamine. Immunology 55: 335–329, 1985Google Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • G. S. Gupta
    • 1
    • 2
  • R. G. Kinsky
    • 1
    • 2
  1. 1.Department of BiophysicsPanjab UniversityChandigarhIndia
  2. 2.INSERM U 262Baudelocque HospitalParisFrance

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