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Rapid cytofluorimetric determination of leaf nuclear DNA content in the polyploid seriesRanunculus marsicus (R. auricomus agg.,Ranunculaceae)

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Abstract

A cytofluorimetric method for DNA amount determination on nuclei released from fixed leaves has been successfully tested on samples belonging to the polyploid series ofRanunculus marsicus Guss & Ten. (sect.Auricomus). The procedure was devised to screen rapidly for different ploidy levels within topodemes and progenies.

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Authors and Affiliations

  1. Dipartimento di Biologia della Università di Milano, via Celoria 26, I-20133, Milano, Italy

    Sergio Sgorbati & Valentina Soler

  2. Dipartimento di Biologia Vegetale dell'Università “La Sapienza” di Roma, Città Universitaria, Roma, Italy

    Stefania M. Masci & Palmer Marchi

Authors
  1. Sergio Sgorbati
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  2. Stefania M. Masci
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  3. Valentina Soler
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  4. Palmer Marchi
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Sgorbati, S., Masci, S.M., Soler, V. et al. Rapid cytofluorimetric determination of leaf nuclear DNA content in the polyploid seriesRanunculus marsicus (R. auricomus agg.,Ranunculaceae). Pl Syst Evol 167, 219–226 (1989). https://doi.org/10.1007/BF00936408

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  • DOI: https://doi.org/10.1007/BF00936408

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