Plant Systematics and Evolution

, Volume 158, Issue 2–4, pp 97–106 | Cite as

Cytochemical heterochromatin differentiation inSinapis alba (Cruciferae) using a simple air-drying technique for producing chromosome spreads

  • Günter Geber
  • Dieter Schweizer
Article

Abstract

The fluorochrome and Giemsa chromosome banding patterns and the Ag-NOR histochemical staining ofSinapis alba are described. Two major types of heterochromatin can be distinguished, one of which contains GC-rich DNA. The observations are discussed as they relate to the known satellite DNAs ofS. alba. — A simple air-drying technique for producing spreads of plant mitotic chromosomes is presented. Different materials and staining techniques were tested showing that the method has wide applications.

Key words

Angiosperms Cruciferae Sinapis alba Chromosome banding chromosome spreading method 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Günter Geber
    • 1
  • Dieter Schweizer
    • 1
  1. 1.Institute of BotanyUniversity of ViennaViennaAustria

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