Molecular and Cellular Biochemistry

, Volume 141, Issue 1, pp 57–63 | Cite as

The relationship of hydrophobic tubulin with membranes in neural tissue

  • Dante M. Beltramo
  • Mariana Nuñez
  • Alejandra del C. Alonso
  • Héctor S. Barra
Article

Abstract

Brain membrane preparations contain tubulin that can be extracted with Triton X-114. After the extract is allowed to partition, 8% of the total brain tubulin is isolated as a hydrophobic compound in the detergent-rich phase. Cytosolic tubulin does not show this hydrophobic behaviour since it is recovered in the aqueous phase. Membrane tubulin can be released by 0.1 M Na2CO3 treatment at pH≥11.5 in such a way that the hydrophobic tubulin is converted into the hydrophilic form. These results suggest that tubulin exists associated with some membrane component that confers the hydrophobic behaviour to tubulin. If the tissue is homogenized in microtubule-stabilizing buffer containing Triton X-100, the hydrophobic tubulin is isolated from the microtubule fraction. This result indicates that the hydrophobic tubulin isolated from membrane preparations belongs to microtubules thatin vivo are associated to membranes. Therefore, hydrophobic tubulin (tubulin-membrane component complex) can be obtained from membranes or from microtubules depending on the conditions of brain homogenization.

Key words

hydrophobic tubulin membrane tubulin tubulin microtubules 

Abbreviations

TBS

Tris-buffered saline

Mes

2-(N-morpholine) ethane sulfonic acid

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Dante M. Beltramo
    • 1
  • Mariana Nuñez
    • 1
  • Alejandra del C. Alonso
    • 1
  • Héctor S. Barra
    • 1
  1. 1.Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), Facultad de Ciencias QuimicasUniversidad Nacional de CórdobaCórdobaArgentina

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