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Glucocorticoids regulate the secretion of a 21kDa-IGF-binding protein by sheep adipose tissue explants

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Abstract

Using a solution phase assay we have demonstrated that sheep adipose tissue explants secrete insulin-like growth factor binding proteins (IGFBPs) when cultured in serum-free medium over a 24 h period. Further, we demonstrate that secretion of IGFBP(s) is inhibited (up to 50%) by incubation of the cultures in the presence of 10−8M dexamethasone. This inhibitory effects is overcome when insulin (10 ng/ml) and ovine growth hormone (100 ng/ml) are incubated together (but not separately) with glucocorticoid. Further characterisation of this IGF binding activity by high performance size exclusion chromatography and Western ligand blot analysis indicated that under our culture conditions sheep adipose tissue explants secrete one predominant 21 kDa IGFBP and it is this BP which is hormonally regulated as described above. We discuss our results in the context of endocrine/paracrine/autocrine control of adipose tissue metabolism and differentiation.

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Abbreviations

IGF:

insulin-like growth factor

IGF-BP:

insulin-like growth factor binding proteins

DX:

dexamethasone

GH:

ovine growth hormone

CM:

conditioned medium

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Authors and Affiliations

  1. Hannah Research Institute, KA6 5HL, Ayr, Scotland, UK

    J. Beattie & R. G. Vernon

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  1. J. Beattie
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  2. R. G. Vernon
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Beattie, J., Vernon, R.G. Glucocorticoids regulate the secretion of a 21kDa-IGF-binding protein by sheep adipose tissue explants. Mol Cell Biochem 145, 151–157 (1995). https://doi.org/10.1007/BF00935487

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  • DOI: https://doi.org/10.1007/BF00935487

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