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Enrichment ofBabesia caballi-infected erythrocytes from microaerophilous stationary-phase cultures using Percoll gradients

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Abstract

A rapid and simple method for concentrating leucocyte-freeBabesia caballi-infected erythrocytes from in vitro cultures is described. Infected erythrocytes amounted to at least 95% of all red cells obtained.

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References

  • Andrysiak PM, Collins WE, Campbell GH (1986) Concentration ofPlasmodium ovale- andPlasmodium vivax-infected erythrocytes from nonhuman primate blood using Percoll gradients. Am J Trop Med Hyg 35:251–254

    Google Scholar 

  • Cissoko A (1989) In vitro-Kultur erythrozytärer Stadien vonBabesia equi undBabesia caballi sowie Gewinnung von Antigenen für Serotests. Thesis. School of Veterinary Medicine, Hanover, Federal Republic of Germany

    Google Scholar 

  • Kramer KJ, Kan SC, Siddiqui WA (1982) Concentration ofPlasmodium falciparum-infected erythrocytes by density gradient centrifugation in Percoll. J Parasitol 68:336–337

    Google Scholar 

  • Levy MG, Ristic M (1980)Babesia bovis: continuous cultivation in a microaerophilous stationary phase culture. Science 207:1218–1220

    Google Scholar 

  • Mahoney DF (1967) Bovine babesiosis: preparation and assessment of complement fixing antigens. Exp Parasitol 20:232–241

    Google Scholar 

  • Pertoft H, Rubin K, Kjellen L, Laurent TC, Klingborn B (1977) The viability of cells grown or centrifuged in a new density gradient medium, Percoll. Exp Cell Res 110:449–457

    Google Scholar 

  • Richards WHG, Williams SG (1973) The removal of leucocytes from malaria-infected blood. Ann Trop Med Parasitol 67:249–250

    Google Scholar 

  • Vega CA, Buening GM, Rodriguez SD, Carson CA (1986) Concentration and enzyme content of in vitro culturedBabesia bigemina-infected erythrocytes. J Protozool 33:514–518

    Google Scholar 

  • Watkins RG (1962) A concentration and staining technique for diagnosing equine piroplasmosis. J Am Vet Med Assoc 141:1330–1332

    Google Scholar 

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Authors and Affiliations

  1. Institut für Parasitologie, Bünteweg 17, W-3000, Hannover 71, Federal Republic of Germany

    C. Bhushan, I. Müller & K. T. Friedhoff

Authors
  1. C. Bhushan
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  2. I. Müller
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  3. K. T. Friedhoff
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Bhushan, C., Müller, I. & Friedhoff, K.T. Enrichment ofBabesia caballi-infected erythrocytes from microaerophilous stationary-phase cultures using Percoll gradients. Parasitol Res 77, 177–179 (1991). https://doi.org/10.1007/BF00935433

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  • DOI: https://doi.org/10.1007/BF00935433

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